HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Cdc42 localization and cell polarity depend on membrane traffic

Abstract : Cell polarity is essential for cell division, cell differentiation, and most differentiated cell functions including cell migration. The small G protein Cdc42 controls cell polarity in a wide variety of cellular contexts. Although restricted localization of active Cdc42 seems to be important for its distinct functions, mechanisms responsible for the concentration of active Cdc42 at precise cortical sites are not fully understood. In this study, we show that during directed cell migration, Cdc42 accumulation at the cell leading edge relies on membrane traffic. Cdc42 and its exchange factor βPIX localize to intracytosplasmic vesicles. Inhibition of Arf6-dependent membrane trafficking alters the dynamics of Cdc42-positive vesicles and abolishes the polarized recruitment of Cdc42 and βPIX to the leading edge. Furthermore, we show that Arf6-dependent membrane dynamics is also required for polarized recruitment of Rac and the Par6–aPKC polarity complex and for cell polarization. Our results demonstrate influence of membrane dynamics on the localization and activation of Cdc42 and consequently on directed cell migration.
Document type :
Journal articles
Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-03521591
Contributor : Florent Peglion Connect in order to contact the contributor
Submitted on : Tuesday, January 11, 2022 - 4:31:43 PM
Last modification on : Thursday, April 7, 2022 - 10:10:52 AM
Long-term archiving on: : Tuesday, April 12, 2022 - 7:47:49 PM

Identifiers

Citation

Naël Osmani, Florent Peglion, Philippe Chavrier, Sandrine Etienne-Manneville. Cdc42 localization and cell polarity depend on membrane traffic. Journal of Cell Biology, Rockefeller University Press, 2010, 191 (7), pp.1261-1269. ⟨10.1083/jcb.201003091⟩. ⟨pasteur-03521591⟩

Share

Metrics

Record views

17

Files downloads

13