Skip to Main content Skip to Navigation
Journal articles

Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity

Abstract : Sulfs represent a class of unconventional sulfatases which provide an original post-synthetic regulatory mechanism for heparan sulfate polysaccharides and are involved in multiple physiopathological processes, including cancer. However, Sulfs remain poorly characterized enzymes, with major discrepancies regarding their in vivo functions. Here we show that human SuIf-2 (HSulf-2) harbors a chondroitin/dermatan sulfate glycosaminoglycan (GAG) chain, attached to the enzyme substrate-binding domain. We demonstrate that this GAG chain affects enzyme/substrate recognition and tunes HSulf-2 activity in vitro and in vivo. In addition, we show that mammalian hyaluronidase acts as a promoter of HSulf-2 activity by digesting its GAG chain. In conclusion, our results highlight HSulf-2 as a proteoglycan-related enzyme and its GAG chain as a critical non-catalytic modulator of the enzyme activity. These findings contribute to clarifying the conflicting data on the activities of the Sulfs.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03637587
Contributor : Nathalie GON Connect in order to contact the contributor
Submitted on : Wednesday, April 13, 2022 - 3:15:15 PM
Last modification on : Friday, August 5, 2022 - 3:44:48 PM
Long-term archiving on: : Thursday, July 14, 2022 - 6:45:18 PM

File

PIIS2211124722002522.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Rana El Masri, Amal Seffouh, Caroline Roelants, Ilham Seffouh, Evelyne Gout, et al.. Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity. Cell Reports, Elsevier Inc, 2022, 38 (11), pp.110516. ⟨10.1016/j.celrep.2022.110516⟩. ⟨hal-03637587⟩

Share

Metrics

Record views

50

Files downloads

10