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DIFFERENTIAL ROBUSTNESS TO SPECIFIC POTASSIUM CHANNEL DELETIONS IN MIDBRAIN DOPAMINERGIC NEURONS

Abstract : Quantifying the level of robustness of neurons in ion channel knock-out (KO) mice depends on how exhaustively electrical phenotype is assessed. We characterized the variations in behavior and electrical phenotype of substantia nigra pars compacta (SNc) dopaminergic neurons in SK3 and Kv4.3 potassium channel KOs. SK3 and Kv4.3 KO mice exhibited a slight increase in exploratory behavior and impaired motor learning, respectively. Combining current-clamp characterization of 16 electrophysiological parameters and multivariate analysis, we found that the electrical phenotype of SK3 KO neurons was not different from wild-type neurons, while that of Kv4.3 KO neurons was significantly altered. Consistently, voltage-clamp recordings of the underlying currents demonstrated that the SK current charge was unchanged in SK3 KO neurons while the Kv4-mediated A-type current was virtually abolished in Kv4.3 KO neurons. We conclude that the robustness of SNc dopaminergic neurons to potassium channel deletions is highly variable, due to channel-specific compensatory mechanisms.
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Preprints, Working Papers, ...
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https://hal.archives-ouvertes.fr/hal-03026595
Contributor : Marianne Amalric <>
Submitted on : Thursday, November 26, 2020 - 5:20:25 PM
Last modification on : Saturday, November 28, 2020 - 3:21:19 AM
Long-term archiving on: : Saturday, February 27, 2021 - 8:05:30 PM

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Alexis Haddjeri-Hopkins, Béatrice Marqueze-Pouey, Monica Tapia, Fabien Tell, Marianne Amalric, et al.. DIFFERENTIAL ROBUSTNESS TO SPECIFIC POTASSIUM CHANNEL DELETIONS IN MIDBRAIN DOPAMINERGIC NEURONS. 2020. ⟨hal-03026595⟩

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