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Caratterizzazione di metaboliti secondari presenti in varie specie di Cistus (fam. Cistaceae) in Sardegna

Abstract : Cistaceae family is widespread in the Mediterranean regions with several species and istraditionally known as a natural remedy. Cistus genus is present in Sardinia with populations of C.monspeliensis, C. salvifolius, C. albidus and C. creticus subspecies : C. creticus subsp. creticus, C.creticus subsp. corsicus and C. creticus subsp. eriocephalus, but few previous phytochemicalresearches have been reported on Cistus species growing in Sardinia.The aim of this research is to characterize the secondarymetabolites in extracts of differentspecies of Cistus in Sardinia and to evaluate antimicrobial and antioxydant activities. The freshaerial parts of the plants were extracted by using hydro distillation for essential oils and severaltraditional solvent s for the phenolic compounds.The chemical characterization of extracts has beenrealized by means of different chromatographic techniques such as GC/MS, HPLC DAD ESI MSand CL UHP SM/SM. Antimicrobial activity was determined as Minimum Inhibitory Concentra tionby using an agar macrodilution method. Antioxidant activity has been measured by using DPPHassay and it has been verified with EPR.A comparative analysis on the composition of essential oils showed the existence of six differentprofiles. C. cretic us subsp. eriocephalus showed a high amount of manoyl oxide and its isomer. C.salvifolius has pointed out the group of labdans; another consistent percentage is made ofperfumed molecules as ionone and its derivate. Several linear hydrocarbons were produc ed by C.monspeliensis, and the heneicosane was the most represented element. In C. albidus no labdanetype diterpenes were identified. Analysis of C. creticus subsp creticus revealed several oxygenatedsesquiterpenes and labdane type diterpenes, especiall y manoyl oxide. C. creticus subsp. corsicuswas qualitatively very similar to C. creticus subsp. creticus, notably concerning the labdane typecompounds. The analysis of the seven essential oils of Cistus creticus subsp. eriocephalus showinteresting chara cteristics and they would appear divided in two groups with different metabolicprofiles. Among solvent extracts the obtained results allowed the detection of several phenoliccompounds including phenolic acids, monomeric and dimeric flavan 3 ols, flavonolglycosides.They are characterized by a hight percentage of rosmarinic acid and derivatives and ofquercitin and derivatives. C. salvifolius is quantitatively most rich of phenolic compounds. Theextracts exhibited any pronounced differences in their anti microbial activities and revealed thatGram positive bacteria are more sensitive to the Cistus extracts than Gram negative bacteria.None of the extracts showed any noticeable action against Candida species. The extracts showedthat Cistus plants of Sardin ian origin have a greater antioxidant activity.
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Patrizia Monica Mastino. Caratterizzazione di metaboliti secondari presenti in varie specie di Cistus (fam. Cistaceae) in Sardegna. Other. Université Pascal Paoli; Università degli studi della Tuscia (Viterbo, Italie), 2017. Italian. ⟨NNT : 2017CORT0001⟩. ⟨tel-03416378⟩

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