The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress

Fan Yingping (1) , Sylvain Lemeille (2) , Andrés González (3) , Véronique Risoul (1) , Yann Denis (4) , Pierre Richaud (5) , Otmane Lamrabet (1) , Maria F Fillat (3) , Cheng-Cai Zhang (1) , Amel Latifi (1)

1 LCB - Laboratoire de chimie bactérienne
2 Department of Microbiology and Molecular Medicine, CMU
3 Departamento de Bioquímica y Biología Molecular y Celular [Zaragoza]
4 Plate-forme Transcriptome FR3479
5 BVME - Biologie végétale et microbiologie environnementale - UMR7265

Background: The filamentous cyanobacterium Nostoc sp. strain PCC 7120 can fix N2 when combined nitrogen is not available. Furthermore, it has to cope with reactive oxygen species generated as byproducts of photosynthesis and respiration. We have previously demonstrated the synthesis of Ser/Thr kinase Pkn22 as an important survival response of Nostoc to oxidative damage. In this study we wished to investigate the possible involvement of this kinase in signalling peroxide stress and nitrogen deprivation. Results: Quantitative RT-PCR experiments revealed that the pkn22 gene is induced in response to peroxide stress and to combined nitrogen starvation. Electrophoretic motility assays indicated that the pkn22 promoter is recognized by the global transcriptional regulators FurA and NtcA. Transcriptomic analysis comparing a pkn22-insertion mutant and the wild type strain indicated that this kinase regulates genes involved in important cellular functions such as photosynthesis, carbon metabolism and iron acquisition. Since metabolic changes may lead to oxidative stress, we investigated whether this is the case with nitrogen starvation. Our results rather invalidate this hypothesis thereby suggesting that the function of Pkn22 under nitrogen starvation is independent of its role in response to peroxide stress. Conclusions: Our analyses have permitted a more complete functional description of Ser/Thr kinase in Nostoc. We have decrypted the transcriptional regulation of the pkn22 gene, and analysed the whole set of genes under the control of this kinase in response to the two environmental changes often encountered by cyanobacteria in their natural habitat: oxidative stress and nitrogen deprivation.

Keywords: Nostoc; Ser/Thr kinase; Oxidative stress; Nitrogen starvation; Signalling; Microarray; ANABAENA SP PCC-7120; SP STRAIN PCC-7120; IRON STARVATION; SYNECHOCOCCUS-ELONGATUS; HETEROCYST DEVELOPMENT; GENE-EXPRESSION; PROTEIN-KINASES; CROSS-TALK; CYANOBACTERIA; IDENTIFICATION

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]

Fan Yingping, Sylvain Lemeille, Andrés González, Véronique Risoul, Yann Denis, et al.. The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress. BMC Genomics, 2015, pp.1-11. ⟨10.1186/s12864-015-1703-1⟩. ⟨hal-01199598⟩