HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Phage-Based Fluorescent Biosensor Prototypes to Specifically Detect Enteric Bacteria Such as E. coli and Salmonella enterica Typhimurium

Abstract : Water safety is a major concern for public health and for natural environment preservation. We propose to use bacteriophages to develop biosensor tools able to detect human and animal pathogens present in water. For this purpose, we take advantage of the highly discriminating properties of the bacteriophages, which specifically infect their bacterial hosts. The challenge is to use a fluorescent reporter protein that will be synthesized, and thus detected, only once the specific recognition step between a genetically modified temperate bacteriophage and its bacterial host has occurred. To ensure the accuracy and the execution speed of our system, we developed a test that does not require bacterial growth, since a simple 1-hour infection step is required. To ensure a high sensitivity of our tool and in order to detect up to a single bacterium, fluorescence is measured using a portable flow cytometer, also allowing on-site detection. In this study, we have constructed and characterized several "phagosensor" prototypes using the HK620 bacteriophage and its host Escher-ichia coli TD2158 and we successfully adapted this method to Salmonella detection. We show that the method is fast, robust and sensitive, allowing the detection of as few as 10 bacteria per ml with no concentration nor enrichment step. Moreover, the test is functional in sea water and allows the detection of alive bacteria. Further development will aim to develop phagosensors adapted on demand to the detection of any human or animal pathogen that may be present in water.
Document type :
Journal articles
Complete list of metadata

Cited literature [44 references]  Display  Hide  Download

Contributor : Administrateur Hal Amu Connect in order to contact the contributor
Submitted on : Thursday, September 17, 2015 - 2:43:58 PM
Last modification on : Tuesday, January 4, 2022 - 6:00:02 PM
Long-term archiving on: : Tuesday, December 29, 2015 - 8:13:37 AM


Publisher files allowed on an open archive



Manon Vinay, Nathalie Franche, Gérald Grégori, Jean-Raphaël Fantino, Flavie Pouillot, et al.. Phage-Based Fluorescent Biosensor Prototypes to Specifically Detect Enteric Bacteria Such as E. coli and Salmonella enterica Typhimurium. PLoS ONE, Public Library of Science, 2015, 10 (7), pp.1-17. ⟨10.1371/journal.pone.0131466⟩. ⟨hal-01201336⟩



Record views


Files downloads