Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections

Nicolas Salez (1) , Astrid Vabret (2) , Marianne Leruez-Ville (3, 4) , Laurent Andreoletti (5) , Fabrice Carrat (6, 7) , Fanny Renois (5) , Xavier de Lamballerie (1, 8)

1 EPV - Emergence des Pathologies Virales
2 Laboratoire de Virologie Humaine et Moléculaire [Caen]
3 Infections à Vih, Réservoirs, Pharmacologie des Antirétroviraux et Prévention de la Transmission Mère Enfant
4 Laboratoire de Virologie
5 CardioVir - Laboratoire de Virologie Médicale et Moléculaire - EA 4684
6 DS3 - ESIM - Déterminants Sociaux de la Santé et du Recours aux Soins
7 CHU Saint-Antoine [AP-HP]
8 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection

Acute Respiratory Infections (ARIs) are responsible for considerable morbidity and mortality worldwide. Documentation of respiratory specimens can help for an appropriate clinical management with a significant effect on the disease progress in patient, the antimicrobial therapy used and the risk of secondary spread of infection. Here, we compared the performances of four commercial multiplex kits used in French University Hospital diagnostic microbiology laboratories for the detection of ARI pathogens (i.e., the xTAG Respiratory Viral Panel Fast, RespiFinder SMART 22, CLART PneumoVir and Fast Track Diagnostics Respiratory Pathogen 33 kits). We used a standardised nucleic acids extraction protocol and a comprehensive comparative approach that mixed reference to well established real-time PCR detection techniques and analysis of convergent positive results. We tested 166 respiratory clinical samples and identified a global high degree of correlation for at least three of the techniques (xTAG, RespiFinder and FTD33). For these techniques, the highest Youden's index (YI), positive predictive (PPV) and specificity (Sp) values were observed for Core tests (e.g., influenza A [YI:0.86-1.00; PPV:78.95-100.00; Sp:97.32-100.00] & B [YI:0.44-1.00; PPV:100.00; Sp:100.00], hRSV [YI:0.50-0.99; PPV:85.71-100.00; Sp:99.38-100.00], hMPV [YI:0.71-1.00; PPV:83.33-100.00; Sp:99.37-100.00], EV/hRV [YI:0.62-0.82; PPV:93.33-100.00; Sp:94.48-100.00], AdV [YI:1.00; PPV:100.00; Sp:100.00] and hBoV [YI:0.20-0.80; PPV:57.14-100.00; Sp:98.14-100.00]). The present study completed an overview of the multiplex techniques available for the diagnosis of acute respiratory infections.

Mots clés: ACID AMPLIFICATION TESTS; VIRAL PANEL ASSAY; REAL-TIME PCR; FILMARRAY RP; XTAG RVP; VIRUS DETECTION; PERFORMANCE; PATHOGENS

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Maladies infectieuses

Nicolas Salez, Astrid Vabret, Marianne Leruez-Ville, Laurent Andreoletti, Fabrice Carrat, et al.. Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections. PLoS ONE, 2015, 10 (6), pp.e0130378. ⟨10.1371/journal.pone.0130378⟩. ⟨hal-01202047⟩