Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry - Archive ouverte HAL Access content directly
Journal Articles Optics Express Year : 2015

Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry

Pierre Bon
Julien Savatier
  • Function : Author
Serge Monneret

Abstract

We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system combines quantitative phase images with varying polarization excitation to create retardance images. This technique is compatible with living samples and gives information about the local retardance and structure of their anisotropic components. We applied our approach to collagen fibers leading to a birefringence value of (3.4 +/- 0.3) . 10(-3) and to living cells, showing that cytoskeleton can be imaged label-free.
Fichier principal
Vignette du fichier
oe-23-12-16383.pdf (4.14 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-01203256 , version 1 (22-09-2015)

Identifiers

  • HAL Id : hal-01203256 , version 1

Cite

Sherazade Aknoun, Pierre Bon, Julien Savatier, Benoit Wattellier, Serge Monneret. Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry. Optics Express, 2015, 23 (12), pp.16383-16406. ⟨hal-01203256⟩
595 View
392 Download

Share

Gmail Facebook Twitter LinkedIn More