Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry

Sherazade Aknoun 1, 2 Pierre Bon 2, 3 Julien Savatier 2 Benoit Wattellier 1 Serge Monneret 2
1 PHASICS SA, XTEC
2 MOSAIC - MOSAIC
FRESNEL - Institut FRESNEL
3 LP2N - Laboratoire Photonique, Numérique et Nanosciences
Abstract : We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system combines quantitative phase images with varying polarization excitation to create retardance images. This technique is compatible with living samples and gives information about the local retardance and structure of their anisotropic components. We applied our approach to collagen fibers leading to a birefringence value of (3.4 +/- 0.3) . 10(-3) and to living cells, showing that cytoskeleton can be imaged label-free.
Keywords : REFRACTIVE-INDEX LIVING CELLS TRANSPARENT RESOLUTION MATRICES POLARIZED PHASE MICROSCOPY DIGITAL HOLOGRAPHIC MICROSCOPY COLLAGEN BIREFRINGENCE OPTICAL COHERENCE TOMOGRAPHY
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Submitted on : Tuesday, September 22, 2015 - 3:44:38 PM
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Sherazade Aknoun, Pierre Bon, Julien Savatier, Benoit Wattellier, Serge Monneret. Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry. Optics Express, Optical Society of America, 2015, 23 (12), pp.16383-16406. ⟨hal-01203256⟩

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