Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry

Aurore Chevin (1) , Bruno Coutard (2) , Philippe Blanchard (1) , Anne-Sophie Dabert-Gay (3) , Magali Ribière-Chabert (1) , Richard Thiéry (1)

1 Laboratoire de Sophia Antipolis
2 AFMB - Architecture et fonction des macromolécules biologiques
3 IPMC - Institut de pharmacologie moléculaire et cellulaire

Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed.

Keywords: ARGININE-RICH MOTIF; BROMOVIRUS CAPSID PROTEIN; HONEYBEE APIS-MELLIFERA; PHYLOGENETIC ANALYSIS; MATURATION CLEAVAGE; SECONDARY STRUCTURE; INFECTED-CELLS; PCR; IDENTIFICATION; LOCALIZATION

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Aurore Chevin, Bruno Coutard, Philippe Blanchard, Anne-Sophie Dabert-Gay, Magali Ribière-Chabert, et al.. Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry. Viruses, 2015, 7 (6), pp.3329-44. ⟨10.3390/v7062774⟩. ⟨hal-01207121⟩