Journal articles
mRNA maturation in giant viruses: variation on a theme
Abstract : Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene cluster both encode the enzyme responsible for the hairpin cleavage and the viral polyA polymerases (vPAP). Unexpectedly, the vPAPs are homodimeric and uniquely self-processive. The vPAP backbone structures exhibit a symmetrical architecture with two subdomains sharing a nucleotidyltransferase topology, suggesting that vPAPs originate from an ancestral duplication. A Poxvirus processivity factor homologue encoded by Megavirus chilensis displays a conserved 5'-GpppA 2'O methyltransferase activity but is also able to internally methylate the mRNAs' polyA tails. These findings elucidate how the arm wrestling between hosts and their viruses to access the translation machinery is taking place in Mimiviridae.
Cited literature [63 references]
https://hal-amu.archives-ouvertes.fr/hal-01214084
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, October 9, 2015 - 5:15:58 PM
Last modification on : Monday, March 29, 2021 - 3:28:02 PM
Long-term archiving on: : Sunday, January 10, 2016 - 10:35:48 AM
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, October 9, 2015 - 5:15:58 PM
Last modification on : Monday, March 29, 2021 - 3:28:02 PM
Long-term archiving on: : Sunday, January 10, 2016 - 10:35:48 AM
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