Skip to Main content Skip to Navigation
Journal articles

mRNA maturation in giant viruses: variation on a theme

Abstract : Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene cluster both encode the enzyme responsible for the hairpin cleavage and the viral polyA polymerases (vPAP). Unexpectedly, the vPAPs are homodimeric and uniquely self-processive. The vPAP backbone structures exhibit a symmetrical architecture with two subdomains sharing a nucleotidyltransferase topology, suggesting that vPAPs originate from an ancestral duplication. A Poxvirus processivity factor homologue encoded by Megavirus chilensis displays a conserved 5'-GpppA 2'O methyltransferase activity but is also able to internally methylate the mRNAs' polyA tails. These findings elucidate how the arm wrestling between hosts and their viruses to access the translation machinery is taking place in Mimiviridae.
Document type :
Journal articles
Complete list of metadatas

Cited literature [63 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01214084
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, October 9, 2015 - 5:15:58 PM
Last modification on : Wednesday, August 19, 2020 - 12:08:10 PM
Long-term archiving on: : Sunday, January 10, 2016 - 10:35:48 AM

File

ptpmcrender.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Stéphane Priet, Audrey Lartigue, Françoise Debart, Jean-Michel Claverie, Chantal Abergel. mRNA maturation in giant viruses: variation on a theme. Nucleic Acids Research, Oxford University Press, 2015, 43, pp.3776-3788. ⟨10.1093/nar/gkv224⟩. ⟨hal-01214084⟩

Share

Metrics

Record views

398

Files downloads

387