Journal articles
Pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation: the Klebsiella paradigm
Abstract : Background: Various methods are currently used to define species and are based on the phylogenetic marker 16S ribosomal RNA gene sequence, DNA-DNA hybridization and DNA GC content. However, these are restricted genetic tools and showed significant limitations.
Results: In this work, we describe an alternative method to build taxonomy by analyzing the pan-genome composition of different species of the Klebsiella genus. Klebsiella species are Gram-negative bacilli belonging to the large Enterobacteriaceae family. Interestingly, when comparing the core/pan-genome ratio; we found a clear discontinuous variation that can define a new species.
Conclusions: Using this pan-genomic approach, we showed that Klebsiella pneumoniae subsp. ozaenae and Klebsiella pneumoniae subsp. rhinoscleromatis are species of the Klebsiella genus, rather than subspecies of Klebsiella pneumoniae. This pan-genomic analysis, helped to develop a new tool for defining species introducing a quantic perspective for taxonomy.
https://hal-amu.archives-ouvertes.fr/hal-01236715
Contributor : Administrateur Hal Amu <>
Submitted on : Wednesday, December 2, 2015 - 10:27:26 AM
Last modification on : Thursday, March 4, 2021 - 3:08:29 AM
Contributor : Administrateur Hal Amu <>
Submitted on : Wednesday, December 2, 2015 - 10:27:26 AM
Last modification on : Thursday, March 4, 2021 - 3:08:29 AM
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s13062-015-0085-2.pdf
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