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Rad59-Facilitated Acquisition of Y' Elements by Short Telomeres Delays the Onset of Senescence

Abstract : Telomerase-negative yeasts survive via one of the two Rad52-dependent recombination pathways, which have distinct genetic requirements. Although the telomere pattern of type I and type II survivors is well characterized, the mechanistic details of short telomere rearrangement into highly evolved pattern observed in survivors are still missing. Here, we analyze immediate events taking place at the abruptly shortened VII-L and native telomeres. We show that short telomeres engage in pairing with internal Rap1-bound TG 1–3-like tracts present between subtelomeric X and Y9 elements, which is followed by BIR-mediated non-reciprocal translocation of Y9 element and terminal TG 1–3 repeats from the donor end onto the shortened telomere. We found that choice of the Y9 donor was not random, since both engineered telomere VII-L and native VI-R acquired Y9 elements from partially overlapping sets of specific chromosome ends. Although short telomere repair was associated with transient delay in cell divisions, Y9 translocation on native telomeres did not require Mec1-dependent checkpoint. Furthermore, the homeologous pairing between the terminal TG 1–3 repeats at VII-L and internal repeats on other chromosome ends was largely independent of Rad51, but instead it was facilitated by Rad59 that stimulates Rad52 strand annealing activity. Therefore, Y9 translocation events taking place during presenescence are genetically separable from Rad51-dependent Y9 amplification process that occurs later during type I survivor formation. We show that Rad59-facilitated Y9 translocations on X-only telomeres delay the onset of senescence while preparing ground for type I survivor formation.
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Dmitri Churikov, Ferose Charifi, Marie-Noëlle Simon, Vincent Géli. Rad59-Facilitated Acquisition of Y' Elements by Short Telomeres Delays the Onset of Senescence. PLoS Genetics, Public Library of Science, 2014, 10 (e1004736), ⟨10.1371/journal.pgen.1004736⟩. ⟨hal-01243511⟩

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