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Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers

Frédéric Harb 1 Marie-Thérèse Giudici-Orticoni 2, 3 Marianne Guiral 2 Bernard Tinland 1
1 CINaM - Centre Interdisciplinaire de Nanoscience de Marseille
2 BIP - Bioénergétique et Ingénierie des Protéines
3 IMM - Institut de Microbiologie de la Méditerranée
Abstract : We have studied the translational migration of a monotopic membrane protein, the bacterial sulfide quinone reductase (SQR) in supported n-bilayers (1≤n≲500) under the influence of an electric field parallel to the membrane plane. The direction of the migration changes when the charge of the protein changes its sign. Measuring mobilities at different pH enables us to gain experimental physico-chemical data on SQR as its isoelectric point and its estimated oligomeric state (at least trimeric) when inserted in a lipid membrane. Consequently, in addition to the migration study of membrane proteins in a lipid environment, this experimental system, previously used with a transmembrane protein, is thus suitable to define membrane protein properties in conditions approaching the native ones (in the absence of detergent).
Keywords : Living systems: Biomimetic Systems
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Submitted on : Friday, January 13, 2017 - 6:40:18 PM
Last modification on : Wednesday, November 3, 2021 - 4:41:21 AM

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Frédéric Harb, Marie-Thérèse Giudici-Orticoni, Marianne Guiral, Bernard Tinland. Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers. European Physical Journal E: Soft matter and biological physics, EDP Sciences: EPJ, 2016, 39, pp.127 ⟨10.1140/epje/i2016-16127-1⟩. ⟨hal-01435354⟩

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