Heterologous Production and Characterization of Two Glyoxal Oxidases from Pycnoporus cinnabarinus

Abstract : The genome of the white rot fungus Pycnoporus cinnabarinus includes a large number of genes encoding enzymes implicated in lignin degradation. Among these, three genes are predicted to encode glyoxal oxidase, an enzyme previously isolated from Phanerochaete chrysosporium The glyoxal oxidase of P. chrysosporium is physiologically coupled to lignin-oxidizing peroxidases via generation of extracellular H2O2 and utilizes an array of aldehydes and α-hydroxycarbonyls as the substrates. Two of the predicted glyoxal oxidases of P. cinnabarinus, GLOX1 (PciGLOX1) and GLOX2 (PciGLOX2), were heterologously produced in Aspergillus niger strain D15#26 (pyrG negative) and purified using immobilized metal ion affinity chromatography, yielding 59 and 5 mg of protein for PciGLOX1 and PciGLOX2, respectively. Both proteins were approximately 60 kDa in size and N-glycosylated. The optimum temperature for the activity of these enzymes was 50°C, and the optimum pH was 6. The enzymes retained most of their activity after incubation at 50°C for 4 h. The highest relative activity and the highest catalytic efficiency of both enzymes occurred with glyoxylic acid as the substrate. The two P. cinnabarinus enzymes generally exhibited similar substrate preferences, but PciGLOX2 showed a broader substrate specificity and was significantly more active on 3-phenylpropionaldehyde
Type de document :
Article dans une revue
Applied and Environmental Microbiology, American Society for Microbiology, 2016, 82 (16), pp.4867-4875. 〈10.1128/AEM.00304-16〉
Liste complète des métadonnées

Littérature citée [35 références]  Voir  Masquer  Télécharger

https://hal-amu.archives-ouvertes.fr/hal-01446831
Contributeur : Sabine Genet <>
Soumis le : mardi 26 septembre 2017 - 22:34:30
Dernière modification le : samedi 30 septembre 2017 - 01:13:44
Document(s) archivé(s) le : mercredi 27 décembre 2017 - 17:10:38

Fichier

AEMDaou2016_{66547F6A-E0ED-48B...
Accord explicite pour ce dépôt

Identifiants

Collections

Citation

Marianne Daou, François Piumi, Daniel Cullen, Eric Record, Craig Faulds. Heterologous Production and Characterization of Two Glyoxal Oxidases from Pycnoporus cinnabarinus. Applied and Environmental Microbiology, American Society for Microbiology, 2016, 82 (16), pp.4867-4875. 〈10.1128/AEM.00304-16〉. 〈hal-01446831〉

Partager

Métriques

Consultations de la notice

130

Téléchargements de fichiers

15