Investigation of the binding properties of a multi-modular GH45 cellulase using bioinspired model assemblies

Fong M (1) , Berrin Jg (2) , Gabriel Paës (1)

1 FARE - Fractionnement des AgroRessources et Environnement
2 BBF - Biodiversité et Biotechnologie Fongiques

Enzymes degrading plant biomass polymers are widely used in biotechnological applications. Their efficiency can be limited by non-specific interactions occurring with some chemical motifs. In particular, the lignin component is known to bind enzymes irreversibly. In order to determine interactions of enzymes with their substrates, experiments are usually performed on isolated simple polymers which are not representative of plant cell wall complexity. But when using natural plant substrates, the role of individual chemical and structural features affecting enzyme-binding properties is also difficult to decipher. RESULTS: We have designed and used lignified model assemblies of plant cell walls as templates to characterize binding properties of multi-modular cellulases. These three-dimensional assemblies are modulated in their composition using the three principal polymers found in secondary plant cell walls (cellulose, hemicellulose, and lignin). Binding properties of enzymes are obtained from the measurement of their mobility that depends on their interactions with the polymers and chemical motifs of the assemblies. The affinity of the multi-modular GH45 cellulase was characterized using a statistical analysis to determine the role played by each assembly polymer. Presence of hemicellulose had much less impact on affinity than cellulose and model lignin. Depending on the number of CBMs appended to the cellulase catalytic core, binding properties toward cellulose and lignin were highly contrasted.

Mots clés: Affinity; Carbohydrate-binding module; Cellulase; Interaction; Lignocellulose; Model assemblies; Modularity

Domaines: Biodiversité; Biotechnologies

Fong M, Berrin Jg, Gabriel Paës. Investigation of the binding properties of a multi-modular GH45 cellulase using bioinspired model assemblies. Biotechnology for Biofuels, 2016, 9, ⟨10.1186/s13068-016-0428-y⟩. ⟨hal-01450838⟩