Adrenomedullin Blockade Suppresses Growth of Human Hormone-Independent Prostate Tumor Xenograft in Mice - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Clinical Cancer Research Année : 2013

Adrenomedullin Blockade Suppresses Growth of Human Hormone-Independent Prostate Tumor Xenograft in Mice

Françoise Boudouresque
  • Fonction : Auteur
Cyrille Bastide
  • Fonction : Auteur
Asma Tounsi
  • Fonction : Auteur
Zohra Benyahia
  • Fonction : Auteur
Julie Acunzo
  • Fonction : Auteur
Nadège Dussault
Dominique Rossi
  • Fonction : Auteur
Denis Bertin

Résumé

Purpose: To study the role of the adrenomedullin system [adrenomedullin and its receptors (AMR), CLR, RAMP2, and RAMP3] in prostate cancer androgen-independent growth. Experimental Design: Androgen-dependent and -independent prostate cancer models were used to investigate the role and mechanisms of adrenomedullin in prostate cancer hormone-independent growth and tumor-associated angiogenesis and lymphangiogenesis. Results: Adrenomedullin and AMR were immunohistochemically localized in the carcinomatous epithelial compartment of prostate cancer specimens of high grade (Gleason score \textgreater7), suggesting a role of the adrenomedullin systemin prostate cancer growth. We used the androgen-independent Du145 cells, for which we demonstrate that adrenomedullin stimulated cell proliferation in vitro through the cAMP/CRAF/MEK/ERK pathway. The proliferation of Du145 and PC3 cells is decreased by anti-adrenomedullin antibody (alpha AM), supporting the fact that adrenomedullin may function as a potent autocrine/paracrine growth factor for prostate cancer androgen-independent cells. In vivo, alpha AM therapy inhibits the growth of Du145 androgen-independent xenografts and interestingly of LNCaP androgen-dependent xenografts only in castrated animals, suggesting strongly that adrenomedullin might play an important role in tumor regrowth following androgen ablation. Histologic examination of alpha AM-treated tumors showed evidence of disruption of tumor vascularity, with depletion of vascular as well as lymphatic endothelial cells and pericytes, and increased lymphatic endothelial cell apoptosis. Importantly, aAM potently blocks tumor-associated lymphangiogenesis, but does not affect established vasculature and lymphatic vessels in normal adult mice. Conclusions: We conclude that expression of adrenomedullin upon androgen ablation in prostate cancer plays an important role in hormone-independent tumor growth and in neovascularization by supplying/amplifying signals essential for pathologic neoangiogenesis and lymphangiogenesis. (C) 2013 AACR.

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Chimie

Dates et versions

hal-01460433 , version 1 (07-02-2017)

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Citer

Caroline Berenguer-Daize, Françoise Boudouresque, Cyrille Bastide, Asma Tounsi, Zohra Benyahia, et al.. Adrenomedullin Blockade Suppresses Growth of Human Hormone-Independent Prostate Tumor Xenograft in Mice. Clinical Cancer Research, 2013, 19 (22), pp.6138--6150. ⟨10.1158/1078-0432.CCR-13-0691⟩. ⟨hal-01460433⟩
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