Polydisperse Structure Factor Model for understanding the ultrasonic scattering from apoptotic cells

Emilie Franceschini; Philippe Pognonec; Benjamin Guillet; Thierry Pourcher; Groupe d'Auteurs Ieee

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Polydisperse Structure Factor Model for understanding the ultrasonic scattering from apoptotic cells

Emilie Franceschini ¹ Philippe Pognonec ^{2, 3} Benjamin Guillet ^{4, 5, 6} Thierry Pourcher ² Groupe d'Auteurs Ieee

LMA - Laboratoire de Mécanique et d'Acoustique [Marseille]

2 TIRO-MATOs - UMR E4320 - Transporteurs et Imagerie, Radiothérapie en Oncologie et Mécanismes biologiques des Altérations du Tissu Osseux

TIRO-MATOs - UMR E4320 : UMR E4320, SHFJ - Service Hospitalier Frédéric Joliot

3 CNRS - Centre National de la Recherche Scientifique

4 VRCM - Vascular research center of Marseille

5 CERIMED - Centre Européen de Recherche en Imagerie médicale

6 Physiopathologie de l'Endothelium

VRCM - Vascular research center of Marseille

Abstract : Quantitative UltraSound (QUS) techniques for determining the tissue microstructure are promising tools to detect and quantify cell death, and thus monitor the tumor response to therapy. QUS techniques rely on theoretical scattering models to fit the BackScatter Coefficient (BSC) from biological tissues to an estimated BSC using an appropriate model. The model adapted to densely packed cells in tumors is the structure factor model. The aim of this work was to use the polydisperse structure factor model to go further in the understanding of the measured BSCs from cells undergoing apoptosis. Ultrasonic backscatter measurements were performed at frequencies ranging from 10 to 42 MHz on centrifugated colon adenocarcinoma (HT29) cell pellets. The cell pellets were employed as simplified in vitro versions of real tumors. The HT29 cells were exposed for 24 hours to different concentrations of the apoptosis inducer staurosporine (0, 0.50, 0.75 and 1 mu M). A parameter estimation procedure was developed in order to estimate the cell size distribution that could explain the measured BSC from all the studied staurosporine concentrations using the polydisperse structure factor model. This procedure assumes that the volume fractions and the relative impedance contrast between the cells and the surrounding medium are the same for all the drug concentrations. The cell size distributions estimated using our parameter estimation procedure were compared to the true size distribution obtained by flow cytometry and histological image analysis. The estimation procedure results revealed that the polydisperse structure factor model could explain the frequency dependence and amplitude of the measured BSCs. The width factor estimated with the polydisperse structure factor model are equal to 67, 32, 17 and 13 for the staurosporine concentrations of 0, 0.50, 0.75 and 1 mu M, respectively. So the width of the distribution becomes larger (with the decrease of the width factor) when the cell death index increases, as observed from the true cell size distribution obtained from flow cytometry and histology. The relative impedance contrast estimates (=0.26) and the volume fraction of the centrifugated cells (=0.70) seem also to be in a reasonable range of value. This study demonstrates that the polydisperse structure factor model could prove to be an invaluable tool for monitoring cell death process.

Keywords : RESPONSES BACKSCATTERING BIOPHANTOMS

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Directions of work or proceedings

Domain :

Life Sciences [q-bio]

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https://hal-amu.archives-ouvertes.fr/hal-01466436
Contributor : Françoise Dignat-George <>
Submitted on : Monday, February 13, 2017 - 3:27:06 PM
Last modification on : Monday, June 21, 2021 - 3:08:02 PM

Polydisperse Structure Factor Model for understanding the ultrasonic scattering from apoptotic cells

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Polydisperse Structure Factor Model for understanding the ultrasonic scattering from apoptotic cells

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