Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice - Aix-Marseille Université Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2016

Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice

Résumé

Inflammatory cells, an integral component of tumor evolution, are present in Glioblastomas multiforme (GBM). To address the cellular basis and dynamics of the inflammatory microenvironment in GBM, we established an orthotopic syngenic model by grafting GL261-DsRed cells in immunocompetent transgenic LysM-EGFP//CD11c-EYFP reporter mice. We combined dynamic spectral two-photon imaging with multiparametric cytometry and multicolor immunostaining to characterize spatio-temporal distribution, morphology and activity of microglia and blood-derived infiltrating myeloid cells in live mice. Early stages of tumor development were dominated by microglial EYFP + cells invading the tumor, followed by massive recruitment of circulating LysM-EGFP + cells. Fluorescent invading cells were conventional XCR1 + and monocyte-derived dendritic cells distributed in subpopulations of different maturation stages, located in different areas relative to the tumor core. The lethal stage of the disease was characterized by the progressive accumulation of EGFP + /EYFP + monocyte-derived dendritic cells. This local phenotypic regulation of monocyte subtypes marked a transition in the immune response.
Fichier principal
Vignette du fichier
srep26381.pdf (3.18 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01468773 , version 1 (15-02-2017)

Identifiants

Citer

Clément Ricard, Aurélie Tchoghandjian, Hervé Luche, Pierre Grenot, Dominique Figarella-Branger, et al.. Phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice. Scientific Reports, 2016, 6, pp.26381 - 26381. ⟨10.1038/srep26381⟩. ⟨hal-01468773⟩
97 Consultations
99 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More