Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients

Fadi Bittar (1) , Hervé Richet (2) , Jean-Christophe Dubus , Martine Reynaud-Gaubert (1) , Nathalie Stremler (3) , Jacques Sarles (3) , Didier Raoult (1, 2) , Jean-Marc Rolain (1)

1 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
3 TIMONE - Hôpital de la Timone [CHU - APHM]

Background: There is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (CF) patients.

Keywords: Cystic fibrosis; Polymerase chain reaction; Cloning; Pseudomonas aeruginosa; Sequence databases; Pneumococcus; Anaerobic bacteria; Bacterial pathogens

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Fadi Bittar, Hervé Richet, Jean-Christophe Dubus, Martine Reynaud-Gaubert, Nathalie Stremler, et al.. Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients. PLoS ONE, 2008, 3 (8), ⟨10.1371/journal.pone.0002908⟩. ⟨hal-01477648⟩