Evaluation of a new extraction protocol for yeast identification by mass spectrometry

Frédérique Gouriet (1) , Farida Ghiab (2) , Carine Couderc (1) , Fadi Bittar (1) , Hervé Tissot Dupont (1) , Christophe Flaudrops (1) , Jean-Paul Casalta (1) , Bissoume Sambe-Ba (3) , Bécaye Fall (4) , Didier Raoult (5, 1) , Florence Fenollar (1)

1 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2 TIMONE - Hôpital de la Timone [CHU - APHM]
3 Hôpital Principal de Dakar
4 LAMIH - Laboratoire d'Automatique, de Mécanique et d'Informatique industrielles et Humaines - UMR 8201
5 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection

In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.

Domaines: Mycologie

Frédérique Gouriet, Farida Ghiab, Carine Couderc, Fadi Bittar, Hervé Tissot Dupont, et al.. Evaluation of a new extraction protocol for yeast identification by mass spectrometry. Journal of Microbiological Methods, 2016, 129, pp.61-65. ⟨10.1016/j.mimet.2016.08.001⟩. ⟨hal-01478536⟩