Phenotypic and genotypic properties of Microbacterium yannicii, a recently described multidrug resistant bacterium isolated from a lung transplanted patient with cystic fibrosis in France

Poonam Sharma (1) , Seydina M Diene (2) , Sandrine Thibeaut (1) , Fadi Bittar (1) , Véronique Roux (1) , Carine Gomez (3) , Martine Reynaud-Gaubert (1) , Jean-Marc Rolain (1)

1 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2 HUG - Geneva University Hospital
3 AP‐MM Marseille - Hôpital Nord AP‐MM Marseille, France

Background: Cystic fibrosis (CF) lung microbiota consists of diverse species which are pathogens or opportunists or have unknown pathogenicity. Here we report the full characterization of a recently described multidrug resistant bacterium, Microbacterium yannicii, isolated from a CF patient who previously underwent lung transplantation. Results: Our strain PS01 (CSUR-P191) is an aerobic, rod shaped, non-motile, yellow pigmented, gram positive, oxidase negative and catalase positive bacterial isolate. Full length 16S rRNA gene sequence showed 98.8% similarity with Microbacterium yannicii G72T type strain, which was previously isolated from Arabidopsis thaliana. The genome size is 3.95Mb, with an average G+C content of 69.5%. In silico DNA-DNA hybridization analysis between our Microbacterium yannicii PS01isolate in comparison with Microbacterium testaceum StLB037 and Microbacterium laevaniformans OR221 genomes revealed very weak relationship with only 28% and 25% genome coverage, respectively. Our strain, as compared to the type strain, was resistant to erythromycin because of the presence of a new erm 43 gene encoding a 23S rRNA N-6-methyltransferase in its genome which was not detected in the reference strain. Interestingly, our patient received azithromycin 250 mg daily for bronchiolitis obliterans syndrome for more than one year before the isolation of this bacterium.

Mots clés: Antibiotic resistance; Multidrug resistant bacteria; Microbacterium yannicii

Domaines: Bactériologie

Poonam Sharma, Seydina M Diene, Sandrine Thibeaut, Fadi Bittar, Véronique Roux, et al.. Phenotypic and genotypic properties of Microbacterium yannicii, a recently described multidrug resistant bacterium isolated from a lung transplanted patient with cystic fibrosis in France. BMC Microbiology, 2013, 13 (1), ⟨10.1186/1471-2180-13-97⟩. ⟨hal-01478651⟩