Human RAD52 Captures and Holds DNA Strands, Increases DNA Flexibility, and Prevents Melting of Duplex DNA: Implications for DNA Recombination

Ineke Brouwer (1) , Hongshan Zhang (1) , Andrea Candelli (1) , Davide Normanno (1) , Erwin J G Peterman (1) , Gijs J L Wuite (1) , Mauro Modesti (1)

1 CRCM - Centre de Recherche en Cancérologie de Marseille

Graphical Abstract Highlights d RAD52 binds ssDNA rapidly and tightly using wrapping and bridging modes d RAD52 binding to dsDNA is slower, weaker, and often diffusive d RAD52 changes dsDNA mechanics and intercalates into the double helix d RAD52 prevents DNA melting by clamping DNA strands (M.M.) In Brief Brouwer et al. show that human RAD52 swiftly and tightly wraps ssDNA around itself. With dsDNA, interactions are weaker and diffusive but drastically change DNA mechanics, suggesting double helix intercalation. DNA-bound RAD52 efficiently captures ssDNA in trans. These features seem favorable for strand annealing, clamping, and second-end capture.

Domaines: Sciences du Vivant [q-bio]

Ineke Brouwer, Hongshan Zhang, Andrea Candelli, Davide Normanno, Erwin J G Peterman, et al.. Human RAD52 Captures and Holds DNA Strands, Increases DNA Flexibility, and Prevents Melting of Duplex DNA: Implications for DNA Recombination. Cell Reports, 2017, 18, pp.2845 - 2853. ⟨10.1016/j.celrep.2017.02.068⟩. ⟨hal-01495423⟩