M. Akrim, M. Bally, G. Ball, J. Tommassen, H. Teerink et al., Xcp-mediated protein secretion in Pseudomonas, 1993.

P. V. Liu, Extracellular Toxins of Pseudomonas aeruginosa, Journal of Infectious Diseases, vol.130, issue.Supplement, pp.94-99, 1974.
DOI : 10.1093/infdis/130.Supplement.S94

T. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular cloning: a laboratory manual, 1982.

G. Michel, S. Bleves, G. Ball, A. Lazdunski, and A. Filloux, Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosa, Microbiology, vol.144, issue.12
DOI : 10.1099/00221287-144-12-3379

V. M. Morales, A. Bäckman, and M. Bagdasarian, A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants, Gene, vol.97, issue.1, pp.39-47, 1991.
DOI : 10.1016/0378-1119(91)90007-X

D. N. Nunn and S. Lory, Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W., Journal of Bacteriology, vol.175, issue.14, pp.4375-4382, 1993.
DOI : 10.1128/jb.175.14.4375-4382.1993

L. J. Overbye, M. Sandkvist, and M. Bagdasarian, Genes required for extracellular secretion of enterotoxin are clustered in Vibrio cholerae, Gene, vol.132, issue.1, pp.101-106, 1993.
DOI : 10.1016/0378-1119(93)90520-D

O. Possot and A. P. Pugsley, Molecular characterization of PulE, a protein required for pullulanase secretion, Molecular Microbiology, vol.263, issue.2, pp.287-299, 1994.
DOI : 10.1016/0378-1119(91)90221-V

A. P. Pugsley, Superfamilies of bacterial transport systems with nucleotide binding components, Symp. Soc. Gen. Microbiol, vol.47, pp.223-248, 1992.

A. P. Pugsley, The complete general secretory pathway in gram-negative bacteria, Microbiol. Rev, vol.57, pp.50-108, 1993.

P. J. Reeves, D. Whitcombe, S. Wharam, M. Gibson, G. Allison et al., Molecular cloning and characterization of 13 out genes from Erwinia carotovora subspecies carotovora: genes encoding members of a general secretion pathway (GSP) widespread in Gram-negative bacteria, Molecular Microbiology, vol.153, issue.3, pp.443-456, 1993.
DOI : 10.1016/0378-1119(91)90221-V

I. Reyss and A. P. Pugsley, Five additional genes in thepulC-O operon of the gram-negative bacteriumKlebslella oxytoca UNF5023 which are required for pullulanase secretion, MGG Molecular & General Genetics, vol.14, issue.2-3, pp.176-184, 1990.
DOI : 10.1007/BF00633815

G. P. Salmond and P. J. Reeves, Membrance traffic wardens and protein secretion in Gram-negative bacteria, Trends in Biochemical Sciences, vol.18, issue.1, pp.7-12, 1993.
DOI : 10.1016/0968-0004(93)90080-7

M. Sandkvist, M. Bagdasarian, S. P. Howard, and V. J. Di-rita, Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae, EMBO J, vol.14, pp.1664-1673, 1995.

A. W. Smith and B. H. Iglewski, by electroporation, Nucleic Acids Research, vol.17, issue.24, p.10509, 1989.
DOI : 10.1093/nar/17.24.10509

D. B. Smith and K. S. Johnson, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase, Gene, vol.67, issue.1, 1988.
DOI : 10.1016/0378-1119(88)90005-4

K. K. Stanley and J. P. Luzio, Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins, EMBO J, vol.3, pp.1429-1434, 1984.

J. D. Thomas, P. J. Reeves, and G. P. Salmond, The general secretion pathway of Erwinia carotovora subsp. carotovora: analysis of the membrane topology of OutC and OutF, Microbiology, vol.143, issue.3, pp.713-720, 1997.
DOI : 10.1099/00221287-143-3-713

L. R. Turner, J. Cano-lara, D. N. Nunn, and S. Lory, Mutations in the consensus ATP-binding sites of XcpR and PilB eliminate extracellular protein secretion and pilus biogenesis in Pseudomonas aeruginosa., Journal of Bacteriology, vol.175, issue.16, pp.4962-4969, 1993.
DOI : 10.1128/jb.175.16.4962-4969.1993

J. E. Walker, M. Saraste, M. J. Runswick, and N. J. Gay, Distantly related sequences in the -and -subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold, EMBO J, vol.8, pp.945-951, 1982.

D. E. Woods and P. A. Sokol, Role of Pseudomonas aeruginosa extracellular enzymes in lung disease, Clin. Investig. Med, vol.9, pp.108-112, 1986.

B. Wretlind and O. Pavlovskis, Genetic mapping and characterization of Pseudomonas aeruginosa mutants defective in the formation of extracellular proteins, J. Bacteriol, vol.158, pp.801-808, 1984.

T. L. Yahr, L. M. Mende-mueller, M. B. Friese, and D. W. Frank, Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon., Journal of Bacteriology, vol.179, issue.22, pp.7165-7168, 1997.
DOI : 10.1128/jb.179.22.7165-7168.1997

C. Yanisch-perron, J. Vieira, and J. Messing, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors, Gene, vol.33, issue.1, pp.103-119, 1985.
DOI : 10.1016/0378-1119(85)90120-9

Y. Zhang and J. K. Broome-smith, Correct insertion of a simple eukaryotic plasma-membrane protein into the cytoplasmic membrane of Escherichia coli, Gene, vol.96, issue.1, pp.51-57, 1990.
DOI : 10.1016/0378-1119(90)90340-W