Uranium Interaction with Two Multi-Resistant Environmental Bacteria: Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris

Isabelle Llorens (1) , Guillaume Untereiner (2) , Danielle Jaillard (3) , Barbara Gouget (4) , Virginie Chapon (5, 6) , Marie Carrière (7)

1 NRS - Nanostructures et Rayonnement Synchrotron
2 LSDRM - Laboratoire Structure et Dynamique par Résonance Magnétique (LCF)
3 MET - Microscopie Électronique
4 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
5 IPM - Interactions Protéine Métal
6 BVME - Biologie végétale et microbiologie environnementale - UMR7265
7 LAN - Laboratoire Lésions des Acides Nucléiques

Depending on speciation, U environmental contamination may be spread through the environment or inversely restrained to a limited area. Induction of U precipitation via biogenic or non-biogenic processes would reduce the dissemination of U contamination. To this aim U oxidation/reduction processes triggered by bacteria are presently intensively studied. Using X-ray absorption analysis, we describe in the present article the ability of Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris, highly resistant to a variety of metals and metalloids or to organic pollutants, to withstand high concentrations of U and to immobilize it either through biosorption or through reduction to non-uraninite U(IV)-phosphate or U(IV)-carboxylate compounds. These bacterial strains are thus good candidates for U bioremediation strategies, particularly in the context of multi-pollutant or mixed-waste contaminations. Citation: Llorens I, Untereiner G, Jaillard D, Gouget B, Chapon V, et al. (2012) Uranium Interaction with Two Multi-Resistant Environmental Bacteria: Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris.

Keywords: uranium; cupriavidus; rhodopseudomonas; Phosphates; Oxygen; Bioremediation; Ecological remediation; Absorption spectroscopy; Gram negative bacteria; Pollutants

Domains: Life Sciences [q-bio]

Isabelle Llorens, Guillaume Untereiner, Danielle Jaillard, Barbara Gouget, Virginie Chapon, et al.. Uranium Interaction with Two Multi-Resistant Environmental Bacteria: Cupriavidus metallidurans CH34 and Rhodopseudomonas palustris. PLoS ONE, 2012, 7 (12), pp.e51783. ⟨10.1371/journal.pone.0051783⟩. ⟨hal-01560936⟩