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Escherichia coli Response to Uranyl Exposure at Low pH and Associated Protein Regulations

Arbia Khemiri 1 Marie Carrière 2 Nicolas Bremond 1, 3 Mohamed Amine Ben Mlouka 4 Laurent Coquet 4 Isabelle Llorens 5 Virginie Chapon 1, 3 Thierry Jouenne 4 Pascal Cosette 4 C. Berthomieu 1, 3
2 LAN - Laboratoire Lésions des Acides Nucléiques
SCIB - UMR E3 - Service de Chimie Inorganique et Biologique : DSM/INAC/SCIB
3 IPM - Interactions Protéine Métal
BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) : DRF/BIAM
4 Plate-forme de Protéomique PISSARO
IRIB - Institute for Research and Innovation in Biomedicine, HeRacLeS - High-tech Research Infrastructures for Life Sciences
5 NRS - Nanostructures et Rayonnement Synchrotron
MEM - Modélisation et Exploration des Matériaux : DRF/IRIG/MEM
Abstract : Better understanding of uranyl toxicity in bacteria is necessary to optimize strains for bioremediation purposes or for using bacteria as biodetectors for bioavailable uranyl. In this study, after different steps of optimization, Escherichia colicells were exposed to uranyl at low pH to minimize uranyl precipitation and to increase its bioavailability. Bacteria were adapted to mid acidic pH before exposure to 50 or 80 mM uranyl acetate for two hours at pH<3. To evaluate the impact of uranium, growth in these conditions were compared and the same rates of cells survival were observed in control and uranyl exposed cultures. Additionally, this impact was analyzedby two-dimensional differential gel electrophoresis proteomics to discover protein actors specifically present or accumulated in contact with uranium.Exposure to uranium resulted in differential accumulation of proteins associated with oxidative stress and in the accumulation of the NADH/quinone oxidoreductase WrbA. This FMN dependent protein performs obligate two-electron reduction of quinones, and may be involved in cells response to oxidative stress. Interestingly, this WrbA protein presents similarities with the chromate reductase from E. coli, which was shown to reduce uranyl in vitro.
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Arbia Khemiri, Marie Carrière, Nicolas Bremond, Mohamed Amine Ben Mlouka, Laurent Coquet, et al.. Escherichia coli Response to Uranyl Exposure at Low pH and Associated Protein Regulations. PLoS ONE, Public Library of Science, 2014, 9 (2), pp.e89863. ⟨10.1371/journal.pone.0089863⟩. ⟨hal-01560952⟩



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