Skip to Main content Skip to Navigation
Journal articles

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Abstract : Currently there is considerable interest in creating ordered arrays of adhesive protein islands in a sea of passivated surface for cell biological studies. In the past years, it has become increasingly clear that living cells respond, not only to the biochemical nature of the molecules presented to them but also to the way these molecules are presented. Creating protein micro-patterns is therefore now standard in many biology laboratories; nano-patterns are also more accessible. However, in the context of cell-cell interactions, there is a need to pattern not only proteins but also lipid bilayers. Such dual proteo-lipidic patterning has so far not been easily accessible. We offer a facile technique to create protein nano-dots supported on glass and propose a method to backfill the inter-dot space with a supported lipid bilayer (SLB). From photo-bleaching of tracer fluorescent lipids included in the SLB, we demonstrate that the bilayer exhibits considerable in-plane fluidity. Functionalizing the protein dots with fluorescent groups allows us to image them and to show that they are ordered in a regular hexagonal lattice. The typical dot size is about 800 nm and the spacing demonstrated here is 2 microns. These substrates are expected to serve as useful platforms for cell adhesion, migration and mechano-sensing studies.
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01577346
Contributor : Igor Ozerov <>
Submitted on : Friday, April 27, 2018 - 4:31:43 PM
Last modification on : Friday, November 6, 2020 - 3:11:15 AM

File

2017-jove-protocol-final-hal.p...
Files produced by the author(s)

Identifiers

Collections

Citation

Emmanuelle Benard, Fuwei Pi, Igor Ozerov, Anne Charrier, Kheya Sengupta. Ligand Nano-cluster Arrays in a Supported Lipid Bilayer. Journal of visualized experiments : JoVE, JoVE, 2017, 122 (122), pp.e55060. ⟨10.3791/55060⟩. ⟨hal-01577346⟩

Share

Metrics

Record views

477

Files downloads

333