Localization of a potassium channel gene (kcne1) to 21q22.1-q22.2 by insitu hybridization and somatic-cell hybridization

Christophe Chevillard (1) , Bernard Attali , Florian Lesage , Michel Fontes , Jacques Barhanin , Michel Lazdunski , Marie Geneviève Mattei (2)

1 LCB - Laboratoire de chimie bactérienne
2 IBCP - Institut de biologie et chimie des protéines [Lyon]

K+ channels form a diverse family of membrane-spanning proteins that exhibit various electrophysiological and pharmacological properties (5). They play a prominent role in a wide variety ofbiological functions such as cell growth, osmotic reg-ulation, hormonal secretion, and excitability (5). Among the various K + channel proteins characterized, the KCNEl pro-tein is distinct from the Shaker-type channels in its structure and biophysical properties (for review, see 6). It consists of 129-130 amino acid residues with a single putative transmem-brane domain and directs the expression of a very slow activat-ing voltage-dependent K + current (4, 7), when expressed in Xenopus oocytes. Originally characterized in epithelial cells (7), KCNEl has also been cloned from neonatal heart, uterus, and human Band T lymphocytes (1-3). Its presence in human T lymphocytes suggested that the KCNEl protein could be involved in the T cell activation process (1). In the present work, we mapped the KCNEl gene by in situ hybridization on human metaphase chromosomes and Southern blot analysis of human-Chinese hamster cell hybrids.

Mots clés: Protein; K+ channels; Epithelial cells

Domaines: Microbiologie et Parasitologie

Christophe Chevillard, Bernard Attali, Florian Lesage, Michel Fontes, Jacques Barhanin, et al.. Localization of a potassium channel gene (kcne1) to 21q22.1-q22.2 by insitu hybridization and somatic-cell hybridization. Genomics, 1993, 15 (1), pp.243-245. ⟨10.1006/geno.1993.1051⟩. ⟨hal-01593104⟩