Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies

Illiassou Hamidou Soumana 1 Bernadette Tchicaya 1 Béatrice Loriod 2 Pascal Rihet 2 Anne Geiger 1
1 UMR 177 IRD-CIRAD
2 TAGC - Technologies avancées pour le génôme et la clinique
Abstract : Sodalisglossinidius,oneofthethreetsetseflymaternallyinheritedsymbionts,waspreviouslyshowntofavorflyinfectionbytrypanosomes,theparasitescausinghumansleepingsickness.Amongapopulationoffliestakingatrypanosome-infectedbloodmeal,onlyafewindividualswillacquiretheparasite;theotherswillescapeinfectionandbeconsideredasrefractorytotrypanosomeinfection.TheaimoftheworkwastoinvestigatewhetherflyrefractorinesscouldbeassociatedwithspecificSodalisgeneexpression.ThetranscriptomeofS.glossinidiusharboredbyfliesthatwerefedeitherwithanon-infectedbloodmeal(control)orwithatrypanosome-infectedmealbutthatdidnotdevelopinfectionwereanalyzed,usingmicroarraytechnology,andcompared.Theanalysisusingthemicroarrayprocedureyielded17genesthatwerefoundtohaveasignificantdifferentialexpressionbetweenthetwogroups.Interestingly,allthesegeneswereoverexpressedinself-cured(refractory)flies.Furtheranalysisoffunctionalannotationofthesegenesindicatedthatmostassociatedbiologicalprocesstermswererelatedtometabolicandbiosyntheticprocessesaswellastooxido-reductionmechanisms.Theseresultsevidencetheoccurrenceofmolecularcrosstalkbetweenthedifferentpartners,inducedbythepassageofthetrypanosomesthroughthefly’sguteventhoughtheparasiteswereunabletoestablishinthegutandtodevelopapermanentinfection.
Keywords : Control flies Self-cured tsetse flies Tripartite interactions Sleeping sickness Tsetse-symbiont-trypanosomes
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Article dans une revue
Frontiers in Microbiology, Frontiers Media, 2014, 5, pp.255. 〈10.3389/fmicb.2014.00255〉
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Illiassou Hamidou Soumana, Bernadette Tchicaya, Béatrice Loriod, Pascal Rihet, Anne Geiger. Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies. Frontiers in Microbiology, Frontiers Media, 2014, 5, pp.255. 〈10.3389/fmicb.2014.00255〉. 〈hal-01612141〉

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