Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies
Abstract
Sodalisglossinidius,oneofthethreetsetseflymaternallyinheritedsymbionts,waspreviouslyshowntofavorflyinfectionbytrypanosomes,theparasitescausinghumansleepingsickness.Amongapopulationoffliestakingatrypanosome-infectedbloodmeal,onlyafewindividualswillacquiretheparasite;theotherswillescapeinfectionandbeconsideredasrefractorytotrypanosomeinfection.TheaimoftheworkwastoinvestigatewhetherflyrefractorinesscouldbeassociatedwithspecificSodalisgeneexpression.ThetranscriptomeofS.glossinidiusharboredbyfliesthatwerefedeitherwithanon-infectedbloodmeal(control)orwithatrypanosome-infectedmealbutthatdidnotdevelopinfectionwereanalyzed,usingmicroarraytechnology,andcompared.Theanalysisusingthemicroarrayprocedureyielded17genesthatwerefoundtohaveasignificantdifferentialexpressionbetweenthetwogroups.Interestingly,allthesegeneswereoverexpressedinself-cured(refractory)flies.Furtheranalysisoffunctionalannotationofthesegenesindicatedthatmostassociatedbiologicalprocesstermswererelatedtometabolicandbiosyntheticprocessesaswellastooxido-reductionmechanisms.Theseresultsevidencetheoccurrenceofmolecularcrosstalkbetweenthedifferentpartners,inducedbythepassageofthetrypanosomesthroughthefly’sguteventhoughtheparasiteswereunabletoestablishinthegutandtodevelopapermanentinfection.
Origin : Publication funded by an institution
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