Journal articles
Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies
Abstract : Sodalisglossinidius,oneofthethreetsetseflymaternallyinheritedsymbionts,waspreviouslyshowntofavorflyinfectionbytrypanosomes,theparasitescausinghumansleepingsickness.Amongapopulationoffliestakingatrypanosome-infectedbloodmeal,onlyafewindividualswillacquiretheparasite;theotherswillescapeinfectionandbeconsideredasrefractorytotrypanosomeinfection.TheaimoftheworkwastoinvestigatewhetherflyrefractorinesscouldbeassociatedwithspecificSodalisgeneexpression.ThetranscriptomeofS.glossinidiusharboredbyfliesthatwerefedeitherwithanon-infectedbloodmeal(control)orwithatrypanosome-infectedmealbutthatdidnotdevelopinfectionwereanalyzed,usingmicroarraytechnology,andcompared.Theanalysisusingthemicroarrayprocedureyielded17genesthatwerefoundtohaveasignificantdifferentialexpressionbetweenthetwogroups.Interestingly,allthesegeneswereoverexpressedinself-cured(refractory)flies.Furtheranalysisoffunctionalannotationofthesegenesindicatedthatmostassociatedbiologicalprocesstermswererelatedtometabolicandbiosyntheticprocessesaswellastooxido-reductionmechanisms.Theseresultsevidencetheoccurrenceofmolecularcrosstalkbetweenthedifferentpartners,inducedbythepassageofthetrypanosomesthroughthefly’sguteventhoughtheparasiteswereunabletoestablishinthegutandtodevelopapermanentinfection.
Cited literature [49 references]
https://hal-amu.archives-ouvertes.fr/hal-01612141
Contributor : Lionel Spinelli <>
Submitted on : Friday, October 12, 2018 - 10:21:32 AM
Last modification on : Tuesday, February 11, 2020 - 10:10:29 AM
Long-term archiving on: : Sunday, January 13, 2019 - 12:51:05 PM
Contributor : Lionel Spinelli <>
Submitted on : Friday, October 12, 2018 - 10:21:32 AM
Last modification on : Tuesday, February 11, 2020 - 10:10:29 AM
Long-term archiving on: : Sunday, January 13, 2019 - 12:51:05 PM
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fmicb-05-00255.pdf
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