Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies - Archive ouverte HAL Access content directly
Journal Articles Frontiers in Microbiology Year : 2014

Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies

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Abstract

Sodalisglossinidius,oneofthethreetsetseflymaternallyinheritedsymbionts,waspreviouslyshowntofavorflyinfectionbytrypanosomes,theparasitescausinghumansleepingsickness.Amongapopulationoffliestakingatrypanosome-infectedbloodmeal,onlyafewindividualswillacquiretheparasite;theotherswillescapeinfectionandbeconsideredasrefractorytotrypanosomeinfection.TheaimoftheworkwastoinvestigatewhetherflyrefractorinesscouldbeassociatedwithspecificSodalisgeneexpression.ThetranscriptomeofS.glossinidiusharboredbyfliesthatwerefedeitherwithanon-infectedbloodmeal(control)orwithatrypanosome-infectedmealbutthatdidnotdevelopinfectionwereanalyzed,usingmicroarraytechnology,andcompared.Theanalysisusingthemicroarrayprocedureyielded17genesthatwerefoundtohaveasignificantdifferentialexpressionbetweenthetwogroups.Interestingly,allthesegeneswereoverexpressedinself-cured(refractory)flies.Furtheranalysisoffunctionalannotationofthesegenesindicatedthatmostassociatedbiologicalprocesstermswererelatedtometabolicandbiosyntheticprocessesaswellastooxido-reductionmechanisms.Theseresultsevidencetheoccurrenceofmolecularcrosstalkbetweenthedifferentpartners,inducedbythepassageofthetrypanosomesthroughthefly’sguteventhoughtheparasiteswereunabletoestablishinthegutandtodevelopapermanentinfection.
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hal-01612141 , version 1 (12-10-2018)

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Attribution - CC BY 4.0

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Cite

Illiassou Hamidou Soumana, Bernadette Tchicaya, Béatrice B. Loriod, Pascal Rihet, Anne Geiger. Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies. Frontiers in Microbiology, 2014, 5, pp.255. ⟨10.3389/fmicb.2014.00255⟩. ⟨hal-01612141⟩
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