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Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies

Abstract : Sodalisglossinidius,oneofthethreetsetseflymaternallyinheritedsymbionts,waspreviouslyshowntofavorflyinfectionbytrypanosomes,theparasitescausinghumansleepingsickness.Amongapopulationoffliestakingatrypanosome-infectedbloodmeal,onlyafewindividualswillacquiretheparasite;theotherswillescapeinfectionandbeconsideredasrefractorytotrypanosomeinfection.TheaimoftheworkwastoinvestigatewhetherflyrefractorinesscouldbeassociatedwithspecificSodalisgeneexpression.ThetranscriptomeofS.glossinidiusharboredbyfliesthatwerefedeitherwithanon-infectedbloodmeal(control)orwithatrypanosome-infectedmealbutthatdidnotdevelopinfectionwereanalyzed,usingmicroarraytechnology,andcompared.Theanalysisusingthemicroarrayprocedureyielded17genesthatwerefoundtohaveasignificantdifferentialexpressionbetweenthetwogroups.Interestingly,allthesegeneswereoverexpressedinself-cured(refractory)flies.Furtheranalysisoffunctionalannotationofthesegenesindicatedthatmostassociatedbiologicalprocesstermswererelatedtometabolicandbiosyntheticprocessesaswellastooxido-reductionmechanisms.Theseresultsevidencetheoccurrenceofmolecularcrosstalkbetweenthedifferentpartners,inducedbythepassageofthetrypanosomesthroughthefly’sguteventhoughtheparasiteswereunabletoestablishinthegutandtodevelopapermanentinfection.
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Illiassou Hamidou Soumana, Bernadette Tchicaya, Béatrice Loriod, Pascal Rihet, Anne Geiger. Identification of overexpressed genes in Sodalis glossinidius inhabiting trypanosome-infected self-cured tsetse flies. Frontiers in Microbiology, Frontiers Media, 2014, 5, pp.255. ⟨10.3389/fmicb.2014.00255⟩. ⟨hal-01612141⟩

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