New perspectives in hydrogenase direct electrochemistry

Abstract : Electrochemical studies of hydrogenases, the biological catalysts of H 2 oxidation and production, have proven wrong the old saying that enzymes do not easily transfer electrons to electrodes in the absence of mediators. Many distinct hydrogenases have actually been directly connected to electrodes or particles, for studying their catalytic mechanism or for designing solar fuels catalysts. In this review, we list the electrodes that have proved successful for direct electron transfer to hydrogenases, and we discuss recent results which illustrate new directions in this research field: the study of the biosynthesis of FeFe hydrogenase, the electrochemical characterization of non-standard NiFe-or FeFe hydrogenases, the general discussion of what makes a catalyst better in one particular direction of the reaction, and the elucidation of the molecular mechanism of hydrogenase catalysis by combining electrochemistry and theoretical chemistry, spectroscopy or photochemistry. The electrochem-ical methods described herein will probably prove useful for studying or using other redox enzymes.
Document type :
Journal articles
Liste complète des métadonnées

Cited literature [109 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01614142
Contributor : Laure Azzopardi <>
Submitted on : Monday, April 16, 2018 - 3:50:45 PM
Last modification on : Thursday, May 17, 2018 - 6:52:02 PM

File

01614142 perspectives-hydrogen...
Files produced by the author(s)

Identifiers

Collections

Citation

Matteo Sensi, Melisa Del Barrio, Carole Baffert, Vincent Fourmond, Christophe Léger. New perspectives in hydrogenase direct electrochemistry. Current Opinion in Electrochemistry, Elsevier, 2017, 5 (1), pp.135-145. ⟨10.1016/j.coelec.2017.08.005⟩. ⟨hal-01614142⟩

Share

Metrics

Record views

87

Files downloads

166