Transcriptomics Reveal Several Gene Expression Patterns in the Piezophile Desulfovibrio hydrothermalis in Response to Hydrostatic Pressure

Abstract : RNA-seq was used to study the response of Desulfovibrio hydrothermalis, isolated from a deep-sea hydrothermal chimney on the East-Pacific Rise at a depth of 2,600 m, to various hydrostatic pressure growth conditions. The transcriptomic datasets obtained after growth at 26, 10 and 0.1 MPa identified only 65 differentially expressed genes that were distributed among four main categories: aromatic amino acid and glutamate metabolisms, energy metabolism, signal transduction, and unknown function. The gene expression patterns suggest that D. hydrothermalis uses at least three different adaptation mechanisms, according to a hydrostatic pressure threshold (HP t) that was estimated to be above 10 MPa. Both glutamate and energy metabolism were found to play crucial roles in these mechanisms. Quantitation of the glutamate levels in cells revealed its accumulation at high hydrostatic pressure, suggesting its role as a piezolyte. ATP measurements showed that the energy metabolism of this bacterium is optimized for deep-sea life conditions. This study provides new insights into the molecular mechanisms linked to hydrostatic pressure adaptation in sulfate-reducing bacteria.
Liste complète des métadonnées

Littérature citée [34 références]  Voir  Masquer  Télécharger

https://hal-amu.archives-ouvertes.fr/hal-01628348
Contributeur : Aurélie Bergon <>
Soumis le : vendredi 3 novembre 2017 - 13:40:46
Dernière modification le : jeudi 18 janvier 2018 - 15:16:01

Fichier

journal.pone.0106831.PDF
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Amira Amrani, Aurélie Bergon, Hélène Holota, Christian Tamburini, Marc Garel, et al.. Transcriptomics Reveal Several Gene Expression Patterns in the Piezophile Desulfovibrio hydrothermalis in Response to Hydrostatic Pressure. PLoS ONE, Public Library of Science, 2014, 9 (9), pp.e106831. 〈http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106831〉. 〈10.1371/journal.pone.0106831〉. 〈hal-01628348〉

Partager

Métriques

Consultations de la notice

78

Téléchargements de fichiers

19