Identification of a perinuclear positioning element in human subtelomeres that requires A-type lamins and CTCF

Abstract : The localization of genes within the nuclear space is of paramount importance for proper genome functions. However, very little is known on the cis-acting elements determining subnuclear positioning of chromosome segments. We show here that the D4Z4 human subtelomeric repeat localizes a telomere at the nuclear periphery. This perinuclear activity lies within an 80 bp sequence included within a region known to interact with CTCF and A-type Lamins. We further show that a reduced level of either CTCF or A-type Lamins suppresses the perinuclear activities of D4Z4 and that an array of multimerized D4Z4 sequence, which has lost its ability to bind CTCF and A-type Lamins, is not localized at the periphery. Overall, these findings reveal the existence of an 80 bp D4Z4 sequence that is sufficient to position an adjacent telomere to the nuclear periphery in a CTCF and A-type lamins-dependent manner. Strikingly, this sequence includes a 30 bp GA-rich motif, which binds CTCF and is present at several locations in the human genome.
Type de document :
Article dans une revue
The EMBO journal., 2009, 28, pp.2428 - 2436
Liste complète des métadonnées

Littérature citée [54 références]  Voir  Masquer  Télécharger

https://hal-amu.archives-ouvertes.fr/hal-01663788
Contributeur : Frederique Magdinier <>
Soumis le : jeudi 14 décembre 2017 - 12:21:14
Dernière modification le : mardi 27 mars 2018 - 11:06:01

Fichier

2428.full.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

  • HAL Id : hal-01663788, version 1

Citation

Alexandre Ottaviani, Caroline Schluth-Bolard, Sylvie Rival-Gervier, Amina Boussouar, Delphine Rondier, et al.. Identification of a perinuclear positioning element in human subtelomeres that requires A-type lamins and CTCF. The EMBO journal., 2009, 28, pp.2428 - 2436. 〈hal-01663788〉

Partager

Métriques

Consultations de la notice

66

Téléchargements de fichiers

16