Skip to Main content Skip to Navigation
Journal articles

Rules Governing Selective Protein Carbonylation

Abstract : Background: Carbonyl derivatives are mainly formed by direct metal-catalysed oxidation (MCO) attacks on the amino-acid side chains of proline, arginine, lysine and threonine residues. For reasons unknown, only some proteins are prone to carbonylation. Methodology/Principal Findings: We used mass spectrometry analysis to identify carbonylated sites in: BSA that had undergone in vitro MCO, and 23 carbonylated proteins in Escherichia coli. The presence of a carbonylated site rendered the neighbouring carbonylatable site more prone to carbonylation. Most carbonylated sites were present within hot spots of carbonylation. These observations led us to suggest rules for identifying sites more prone to carbonylation. We used these rules to design an in silico model (available at http://www.lcb.cnrs-mrs.fr/CSPD/), allowing an effective and accurate prediction of sites and of proteins more prone to carbonylation in the E. coli proteome. Conclusions/Significance: We observed that proteins evolve to either selectively maintain or lose predicted hot spots of carbonylation depending on their biological function. As our predictive model also allows efficient detection of carbonylated proteins in Bacillus subtilis, we believe that our model may be extended to direct MCO attacks in all organisms.
Document type :
Journal articles
Complete list of metadatas

Cited literature [48 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01696357
Contributor : Administrateur Hal Amu <>
Submitted on : Tuesday, January 30, 2018 - 12:47:45 PM
Last modification on : Tuesday, March 17, 2020 - 2:53:15 AM
Long-term archiving on: : Friday, May 25, 2018 - 7:38:51 PM

File

journal.pone.0007269.PDF
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Etienne Maisonneuve, Adrien Ducret, Pierre Khoueiry, Sabrina Lignon, Sonia Longhi, et al.. Rules Governing Selective Protein Carbonylation. PLoS ONE, Public Library of Science, 2009, 4 (10), pp.e7269. ⟨10.1371/journal.pone.0007269⟩. ⟨hal-01696357⟩

Share

Metrics

Record views

370

Files downloads

283