Skip to Main content Skip to Navigation
Journal articles

A demonstration of the H3 trimethylation ChIP-seq analysis of galline follicular mesenchymal cells and male germ cells

Abstract : Trimethylation of histone 3 (H3) at 4th lysine N-termini (H3K4me3) in gene promoter region was the universal marker of active genes specific to cell lineage. On the contrary, coexistence of trimethylation at 27th lysine (H3K27me3) in the same loci-the bivalent H3K4m3/H3K27me3 was known to suspend the gene transcription in germ cells, and could also be inherited to the developed stem cell. In galline species, throughout example of H3K4m3 and H3K27me3 ChIP-seq analysis was still not provided. We therefore designed and demonstrated such procedures using ChIP-seq and mRNA-seq data of chicken follicular mesenchymal cells and male germ cells.
Complete list of metadatas

Cited literature [22 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01743254
Contributor : Denis Puthier <>
Submitted on : Thursday, January 10, 2019 - 2:23:05 PM
Last modification on : Thursday, February 7, 2019 - 2:46:09 PM
Long-term archiving on: : Thursday, April 11, 2019 - 3:33:05 PM

File

1K_ChIP-seq.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Kaj Chokeshaiusaha, Denis Puthier, Catherine Nguyen, Thanida Sananmuang. A demonstration of the H3 trimethylation ChIP-seq analysis of galline follicular mesenchymal cells and male germ cells. Asian-Australasian journal of animal sciences, The Asian-australasian Association of Animal, 2018, ⟨10.5713/ajas.17.0744⟩. ⟨hal-01743254⟩

Share

Metrics

Record views

122

Files downloads

207