Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice - Archive ouverte HAL Access content directly
Journal Articles Allergy Year : 2017

Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice

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Abstract

BackgroundImmunomodulatory interventions play a key role in the treatment of infections and cancer as well as allergic diseases. Adjuvants such as micro- and nanoparticles are often added to immunomodulatory therapies to enhance the triggered immune response. Here, we report the immunological assessment of novel and economically manufactured microparticle adjuvants, namely strontium-doped hydroxyapatite porous spheres (SHAS), which we suggest for the use as adjuvant and carrier in allergen-specific immunotherapy (ASIT). Methods and resultsScanning electron microscopy revealed that the synthesis procedure developed for the production of SHAS results in a highly homogeneous population of spheres. Strontium-doped hydroxyapatite porous spheres bound and released proteins such as ovalbumin (OVA) or the major cat allergen Fel d 1. SHAS-OVA were taken up by human monocyte-derived dendritic cells (mdDCs) and murine DCs and did not have any necrotic or apoptotic effects even at high densities. In a murine model of ASIT for allergic asthmatic inflammation, we found that OVA released from subcutaneously injected SHAS-OVA led to a sustained stimulation of both CD4(+) and CD8(+) T cells. Allergen-specific immunotherapy with SHAS-OVA as compared to soluble OVA resulted in similar humoral responses but in a higher efficacy as assessed by symptom scoring. ConclusionWe conclude that SHAS may constitute a suitable carrier and adjuvant for ASIT with great potential due to its unique protein-binding properties.

Dates and versions

hal-01765078 , version 1 (12-04-2018)

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M. Garbani, W. Xia, C. Rhyner, M. Prati, A. Scheynius, et al.. Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice. Allergy, 2017, 72 (4), pp.570-578. ⟨10.1111/all.13041⟩. ⟨hal-01765078⟩
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