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Integrin-mediated human glioblastoma cells adhesion, migration and invasion by native and recombinant phospholipases of Scorpio maurus venom glands

Najeh Krayem 1 Zaineb Abdelkefi-Koubaa 2 Youssef Gargouri 1, * José Luis 3
* Corresponding author
1 Université de Sfax - University of Sfax
2 LR11IPT08 - Laboratoire des Venins et Biomolécules Thérapeutiques - Laboratory of Venoms and Therapeutic Biomolecules
3 CRO2 - Centre de Recherches en Oncologie biologique et Oncopharmacologie
Abstract : Integrins are a large family of cell surface receptors mediating the interaction of cells with their microenvironment and they play an important role in glioma biology. In the present work, we reported the anti-tumor effect of Sm-PLGV a phospholipase A2 from Tunisian scorpion venom glands-as well as its recombinant forms expressed in Escherichia coli-through interference with integrin receptor function in malignant glioma cells U87. These phospholipases inhibited in a dose dependent manner the adhesion, migration and invasion onto fibrinogen and fibronectin without any cytotoxicity. We showed that Sm-PLGV and its recombinant constructs blocked U87 migration by reducing their velocity and directional persistence. The inhibitory effect was related to a blockage of the integrins αvβ3 and α5β1 function. Inactivation of the enzymatic activity of Sm-PLGV by chemical modification with p-bromophenacyl bromide did not affect its anti-tumor properties, suggesting the presence of 'pharmacological sites' distinct from the catalytic site in scorpion venom phospholipases A2.
Keywords : Recombinant phospholipases Scorpion venom glands Integrins Glioblastoma Extracellular matrix
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Journal articles
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https://hal-amu.archives-ouvertes.fr/hal-01765741
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Submitted on : Friday, April 13, 2018 - 9:43:19 AM
Last modification on : Tuesday, June 23, 2020 - 12:30:05 PM

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Najeh Krayem, Zaineb Abdelkefi-Koubaa, Youssef Gargouri, José Luis. Integrin-mediated human glioblastoma cells adhesion, migration and invasion by native and recombinant phospholipases of Scorpio maurus venom glands. Archives of Biochemistry and Biophysics, Elsevier, 2018, 645, pp.19 - 25. ⟨10.1016/j.abb.2018.03.014⟩. ⟨hal-01765741⟩

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