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Oligoesculin fraction induces anti-tumor effects and promotes immune responses on B16-F10 mice melanoma

Abstract : Laccase was used to enzymatically polymerize esculin. Oligoesculin fraction was obtained after ultrafiltration through a 5-kDa membrane. Several studies have been carried out to prove the effectiveness of natural substances such as immunomodulators to promote the anti-cancer activity in situ. The purpose of our report was to explore whether the anti-tumor potential of the oligoesculin fraction in vitro and in vivo is linked to its immunological mechanisms in melanoma-bearing mice. We revealed that oligoesculin fraction reduced B16-F10 proliferation and migration in vitro in a dose-related manner. Moreover, melanin synthesis and tyrosinase activity were inhibited in these melanoma cells in a concentration-dependent way. The anti-tumor potential of oligoesculin fraction was also assessed in vivo. Our results showed that intraperitoneal administration of oligoesculin fraction, at 50 mg/kg body weight (b.w.) for 21 days, reduced tumor size and weight with percentages of inhibition of 94 and 87 %, respectively. Oligoesculin fraction was effective in promoting lysosomal activity and nitric oxide (NO) production by peritoneal macrophages in tumor-implanted mice. In addition, the activities of natural killer (NK), cytotoxic T lymphocytes, and macrophages were significantly enhanced by oligoesculin fraction. These findings suggested that this polymer with its anti-tumor and immunomodulatory properties could be used for the treatment of melanoma.
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https://hal-amu.archives-ouvertes.fr/hal-01765769
Contributor : Ludovic Leloup <>
Submitted on : Friday, April 13, 2018 - 9:55:51 AM
Last modification on : Thursday, June 4, 2020 - 3:50:41 AM

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Imen Mokdad Bzeouich, Nadia Mustapha, Aicha Sassi, Kamel Ghedira, Mohamed Ghoul, et al.. Oligoesculin fraction induces anti-tumor effects and promotes immune responses on B16-F10 mice melanoma. Tumor Biology, Springer Verlag, 2016, 37 (8), pp.11349 - 11358. ⟨10.1007/s13277-016-5011-4⟩. ⟨hal-01765769⟩

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