Skip to Main content Skip to Navigation
Journal articles

A-, T-, and H-type Currents Shape Intrinsic Firing of Developing Rat Abducens Motoneurons

Abstract : During postnatal development, profound changes take place in the excitability of nerve cells, including modification in the distribution and properties of receptor‐operated channels and changes in the density and nature of voltage‐gated channels. We studied here the firing properties of abducens motoneurons (aMns) in transverse brainstem slices from postnatal day (P) 1–13 rats. Recordings were made from aMNs in the whole‐cell configuration of the patch‐clamp technique. Two main types of aMn could be distinguished according to their firing profile during prolonged depolarizations. Both types were identified as aMns by their fluorescence following retrograde labelling with the lipophilic carbocyanine DiI in the rectus lateralis muscle. The first type (BaMns) exhibited a burst of action potentials (APs) followed by an adaptation of discharge and were encountered in ∼70 % of aMns. Their discharge profile resembled that of adult aMns and was encountered in all aMns after P9. BaMns exhibited a hyperpolarization‐induced rebound potential that was blocked by low concentrations of Ni2+ or by Ca2+‐free external solution. This current had the properties of the T‐type current. Action potentials of BaMns showed a complex afterhyperpolarization (AHP). An inward rectification was evidenced following hyperpolarization and was blocked by external application of caesium or ZD7288, indicating the presence of the hyperpolarization‐activated cationic current (IH). Blocking the IH current almost doubled the input resistance of BaMns. The second class of aMns (DaMns) displayed a delayed excitation that was mediated by A‐type K+ currents and was observed only between P4 and P9. DaMns exhibited immature characteristics: an action potential with a simple AHP, a linear current‐voltage relation and a large input resistance. The number of aMns remained unchanged when both types were present (P5–P6) and later in development when only BaMns were encountered (P19), suggesting that DaMns mature into BaMns during postnatal development. We conclude that aMns display profound reorganization in their intrinsic excitability during postnatal development.
Document type :
Journal articles
Complete list of metadatas

Cited literature [45 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01766855
Contributor : Dominique Debanne <>
Submitted on : Monday, April 30, 2018 - 5:00:09 PM
Last modification on : Friday, April 17, 2020 - 2:56:03 PM
Long-term archiving on: : Tuesday, September 18, 2018 - 11:06:42 AM

File

Russier-Debanne-JPhysiol-2003....
Files produced by the author(s)

Identifiers

Collections

Citation

Michaël Russier, Edmond Carlier, Norbert Ankri, Laure Fronzaroli, Dominique Debanne. A-, T-, and H-type Currents Shape Intrinsic Firing of Developing Rat Abducens Motoneurons. The Journal of Physiology, Wiley, 2003, 549 (1), pp.21-36. ⟨10.1113/jphysiol.2002.037069⟩. ⟨hal-01766855⟩

Share

Metrics

Record views

131

Files downloads

229