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All-trans-retinoic acid represses chemokine expression in adipocytes and adipose tissue by inhibiting 1 NF-κB signaling 2 3

Abstract : An effect of the Vitamin A metabolite all-trans-retinoic acid (ATRA) on body weight regulation and adiposity has been described, but little is known about its impact on obesity-associated inflammation. Our objective was to evaluate the overall impact of this metabolite on inflammatory response in human and mouse adipocytes, using high-throughput methods, and to confirm its effects in a mouse model. ATRA (2 μM for 24 h) down-regulated the mRNA expression of 17 chemokines in human adipocytes, and limited macrophage migration in a TNFα-conditioned 3 T3-L1 adipocyte medium (73.7%, P<.05). These effects were confirmed in mice (n=6–9 per group) subjected to oral gavage of ATRA (5 mg/kg of body weight) and subsequently injected intraperitoneally with lipopolysaccharide. In this model, both systemic and adipose levels of inflammatory markers were reduced. The antiinflammatory effect of ATRA was associated with a reduction in the phosphorylation levels of IκB and p65 (~50%, P<.05), two subunits of the NF-κB pathway, probably mediated by PGC1α, in 3 T3-L1 adipocytes. Taken together, these results show a significant overall antiinflammatory effect of ATRA on proinflammatory cytokine and chemokine production in adipocyte and adipose tissue and suggest that ATRA supplementation may represent a strategy of preventive nutrition to fight against obesity and its complications.
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https://hal-amu.archives-ouvertes.fr/hal-01767340
Contributor : Jean-Francois Landrier <>
Submitted on : Monday, April 16, 2018 - 10:21:05 AM
Last modification on : Tuesday, March 17, 2020 - 3:24:28 AM

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  • HAL Id : hal-01767340, version 1

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Esma Karkeni, Lauriane Bonnet, Julien Astier, Charlène Couturier, Julie Dalifard, et al.. All-trans-retinoic acid represses chemokine expression in adipocytes and adipose tissue by inhibiting 1 NF-κB signaling 2 3. Journal of Nutritional Biochemistry, Elsevier, 2017, 42, pp.101-107. ⟨hal-01767340⟩

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