Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Tomas Ekeberg (1) , Martin Kauffmann , M Marvin Seibert , Chantal Abergel (2) , Filipe Maia , Virginie Seltzer (3) , Daniel Deponte , Andrew Aquila (4) , Andreas Andreasson , Bianca Iwan (5) , Olof Jönsson , Daniel Westphal , Duško Odić , Inger Andersson (6) , Anton Barty (7) , Meng Liang (8) , Andrew Martin (9) , Lars Gumprecht , Holger Fleckenstein , Saša Bajt , Miriam Barthelmess , Nicola Coppola (10) , Jean-Michel Claverie (2) , N Duane Loh , Christoph Bostedt (11) , John Bozek (12) , Jacek Krzywinski (13) , Marc Messerschmidt (14) , Michael Bogan , Christina Hampton , Raymond Sierra , Matthias Frank (7) , Robert Shoeman , Lukas Lomb , Lutz Foucar (15) , Sascha Epp , Daniel Rolles (16) , Artem Rudenko (17) , Robert Hartmann , Andreas Hartmann (18) , Nils Kimmel , Peter Holl , Georg Weidenspointner (19) , Benedikt Rudek , Benjamin Erk (16) , Stephan Kassemeyer , Ilme Schlichting (20) , Lothar Strüder , Joachim Ullrich (21) , Carlo Schmidt , Faton Krasniqi , Günter Hauser , Christian Reich , Heike Soltau , Sebastian Schorb (13) , Helmut Hirsemann , Cornelia Wunderer (22) , Heinz Graafsma , Henry Chapman (23) , Janos Hajdu (1) , M. Marvin Seibert , Filipe R.N.C. Maia , N. Duane Loh

1 Department of Cell and Molecular Biology [Uppsala]
2 IGS - Information génomique et structurale
3 UPD7 - Université Paris Diderot - Paris 7
4 LCLS - Linac Coherent Light Source
5 QUEST - Centre for Quantum Engineering and Space-time Research
6 Department of Molecular Biology
7 LLNL - Lawrence Livermore National Laboratory
8 LIAMA - Brain imaging
9 University of Oxford
10 Public Medicine Infectious Diseases Section
11 ANL - Argonne National Laboratory [Lemont]
12 SSOLEIL - Synchrotron SOLEIL
13 SLAC - SLAC National Accelerator Laboratory
14 NSF - National Science Foundation [Arlington]
15 Max Planck Institute for Medical Research [Heidelberg]
16 DESY - Deutsches Elektronen-Synchrotron [Hamburg]
17 Kansas State University
18 CHU Pitié-Salpêtrière [AP-HP]
19 CESR - Centre d'étude spatiale des rayonnements
20 Department of Biomolecular Mechanisms
21 MPIK - Max-Planck-Institut für Kernphysik
22 SSL - Space Sciences Laboratory [Berkeley]
23 DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Structural Biology [q-bio.BM]

Tomas Ekeberg, Martin Kauffmann, M Marvin Seibert, Chantal Abergel, Filipe Maia, et al.. Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser. Scientific Data , 2016, 3, pp.160060. ⟨10.1038/sdata.2016.60⟩. ⟨hal-01768352⟩