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Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Tomas Ekeberg 1 Martin Kauffmann M Marvin Seibert Chantal Abergel 2 Filipe Maia Virginie Seltzer 3 Daniel Deponte Andrew Aquila 4 Andreas Andreasson Bianca Iwan 5 Olof Jönsson Daniel Westphal Duško Odić Inger Andersson 6 Anton Barty 7 Meng Liang 8 Andrew Martin 9 Lars Gumprecht Holger Fleckenstein Saša Bajt Miriam Barthelmess Nicola Coppola 10 Jean-Michel Claverie 2 N Duane Loh Christoph Bostedt 11 John Bozek 12 Jacek Krzywinski 13 Marc Messerschmidt 14 Michael Bogan Christina Hampton Raymond Sierra Matthias Frank 7 Robert Shoeman Lukas Lomb Lutz Foucar 15 Sascha Epp Daniel Rolles 16 Artem Rudenko 17 Robert Hartmann Andreas Hartmann 18 Nils Kimmel Peter Holl Georg Weidenspointner 19 Benedikt Rudek Benjamin Erk 16 Stephan Kassemeyer Ilme Schlichting 20 Lothar Strüder Joachim Ullrich 21 Carlo Schmidt Faton Krasniqi Günter Hauser Christian Reich Heike Soltau Sebastian Schorb 22 Helmut Hirsemann Cornelia Wunderer 23 Heinz Graafsma Henry Chapman 24 Janos Hajdu 1 M. Marvin Seibert Filipe R.N.C. Maia N. Duane Loh
1 Department of Cell and Molecular Biology [Uppsala]
2 IGS - Information génomique et structurale
3 UPD7 - Université Paris Diderot - Paris 7
4 LCLS - Linac Coherent Light Source
5 QUEST - Centre for Quantum Engineering and Space-time Research
6 Department of Molecular Biology
7 LLNL - Lawrence Livermore National Laboratory
8 LIAMA - Brain imaging
9 University of Oxford [Oxford]
10 Public Medicine Infectious Diseases Section
11 ANL - Argonne National Laboratory [Lemont]
12 SSOLEIL - Synchrotron SOLEIL
13 SLAC - Stanford Linear Accelerator Center
14 NSF - National Science Foundation [Arlington]
15 Max Planck Institute for Medical Research [Heidelberg]
16 DESY - Deutsches Elektronen-Synchrotron [Hamburg]
17 Kansas State University
18 Centre de Référence National 'Syndrome Gilles de la Tourette', Pôle des Maladies du Système Nerveux, Groupe Hospitalier Pitié-Salpêtrière, Assistance Publique des Hôpitaux de Paris
19 CESR - Centre d'étude spatiale des rayonnements
20 Department of Biomolecular Mechanisms
21 MPIK - Max-Planck-Institut für Kernphysik
22 SLAC - SLAC National Accelerator Laboratory
23 SSL - Space Sciences Laboratory [Berkeley]
24 DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany
Abstract : Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.
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https://hal-amu.archives-ouvertes.fr/hal-01768352
Contributor : Olivier Poirot <>
Submitted on : Tuesday, April 17, 2018 - 10:45:13 AM
Last modification on : Thursday, October 15, 2020 - 3:36:16 AM

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UNIV-AMU | CNRS | UNIV-PARIS7 | INRA | OMP-IRAP | CIRAD | USPC | SYNCHROTRON-SOLEIL | LIAMA | INRIA | INSU | OMP | UNIV-TLSE3 | IGS | INRAE | UNIV-PARIS | METEO

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Tomas Ekeberg, Martin Kauffmann, M Marvin Seibert, Chantal Abergel, Filipe Maia, et al.. Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser. Scientific Data , Nature Publishing Group, 2016, 3, pp.160060. ⟨10.1038/sdata.2016.60⟩. ⟨hal-01768352⟩

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