Journal articles
Distinguishing Body Lice from Head Lice by Multiplex Real-Time PCR Analysis of the Phum_PHUM540560 Gene
Abstract : Background: Body louse or head louse? Once removed from their environment, body and head lice are indistinguishable. Neither the morphological criteria used since the mid-18th century nor the various genetic studies conducted since the advent of molecular biology tools have allowed body lice and head lice to be differentiated. In this work, using a portion of the Phum_PHUM540560 gene from the body louse, we aimed to develop a multiplex real-time polymerase chain reaction (PCR) assay to differentiate between body and head lice in a single reaction.
Materials and Methods
A total of 142 human lice were collected from mono-infested hosts from 13 countries on five continents. We first identified the louse clade using a cytochrome b (CYTB) PCR sequence alignment. We then aligned a fragment of the Phum_PHUM540560 gene amplified from head and body lice to design-specific TaqMan© FAM- and VIC-labeled probes.
Results: All the analyzed lice were Clade A lice. A total of 22 polymorphisms between the body and head lice were characterized. The multiplex real-time PCR analysis enabled the body and head lice to be distinguished in two hours. This method is simple, with 100% specificity and sensitivity.
Conclusions:We confirmed that the Phum_PHUM540560 gene is a useful genetic marker for the study of lice.
Cited literature [34 references]
https://hal-amu.archives-ouvertes.fr/hal-01771773
Contributor : Administrateur Hal Amu <>
Submitted on : Thursday, April 19, 2018 - 6:10:28 PM
Last modification on : Thursday, July 11, 2019 - 2:10:08 PM
Long-term archiving on: : Tuesday, September 18, 2018 - 3:48:10 PM
Contributor : Administrateur Hal Amu <>
Submitted on : Thursday, April 19, 2018 - 6:10:28 PM
Last modification on : Thursday, July 11, 2019 - 2:10:08 PM
Long-term archiving on: : Tuesday, September 18, 2018 - 3:48:10 PM
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journal.pone.0058088.PDF
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