Skip to Main content Skip to Navigation
Journal articles

Characterisation of the organophosphate hydrolase catalytic activity of SsoPox

Abstract : SsoPox is a lactonase endowed with promiscuous phosphotriesterase activity isolated from Sulfolobus solfataricus that belongs to the Phosphotriesterase-Like Lactonase family. Because of its intrinsic thermal stability, SsoPox is seen as an appealing candidate as a bioscavenger for organophosphorus compounds. A comprehensive kinetic characterisation of SsoPox has been performed with various phosphotriesters (insecticides) and phosphodiesters (nerve agent analogues) as substrates. We show that SsoPox is active for a broad range of OPs and remains active under denaturing conditions. In addition, its OP hydrolase activity is highly stimulated by anionic detergent at ambient temperature and exhibits catalytic efficiencies as high as k cat /K M of 10 5 M 21 s 21 against a nerve agent analogue. The structure of SsoPox bound to the phosphotriester fensulfothion reveals an unexpected and non-productive binding mode. This feature suggests that SsoPox's active site is sub-optimal for phosphotriester binding, which depends not only upon shape but also on localised charge of the ligand.
Document type :
Journal articles
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01772542
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, April 20, 2018 - 1:34:13 PM
Last modification on : Tuesday, April 24, 2018 - 12:14:17 PM
Long-term archiving on: : Tuesday, September 18, 2018 - 9:37:46 PM

File

srep00779.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License

Identifiers

Collections

Citation

Julien Hiblot, Guillaume Gotthard, Eric Chabriere, Mikael Elias. Characterisation of the organophosphate hydrolase catalytic activity of SsoPox. Scientific Reports, Nature Publishing Group, 2012, 2 (1), ⟨10.1038/srep00779⟩. ⟨hal-01772542⟩

Share

Metrics

Record views

286

Files downloads

258