Journal articles
Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases
Abstract : NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases. Here, we describe the development of new assays for measuring the activity of NADPH oxidases enabling the high-throughput screening for NADPH oxidase inhibitors.
Keywords :
amplex red assay
cytochrome-c reductase
extracellular-superoxide
Fluorescent probes
High-throughput screening
hplc
human-neutrophils
hydrogen-peroxide
intracellular superoxide
NADPH oxidase
nitroblue tetrazolium
nox enzymes
reactive oxygen
Reactive oxygen species
superoxide anion production
https://hal-amu.archives-ouvertes.fr/hal-01774183
Contributor : Marlene Delhaye Connect in order to contact the contributor
Submitted on : Monday, April 23, 2018 - 2:41:39 PM
Last modification on : Friday, January 7, 2022 - 3:53:39 AM
Contributor : Marlene Delhaye Connect in order to contact the contributor
Submitted on : Monday, April 23, 2018 - 2:41:39 PM
Last modification on : Friday, January 7, 2022 - 3:53:39 AM
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