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G. Cacggtatccccgg-kd4-nt-pkd4, C. Tgttcaccg-kd4-nt-pkd4, and C. Gctcatccatgccg-kd4-ct, KD4-Ct-mCherry-3 insertion of mCherry into pKD4 CTTCGAAGCAGCTCCAGCCTACACTTACTTGTACAGCTCGTCCATGCC GCC 5-pETG20-TssM 26Ct insertion of tssM (aa 868-1107) into pETG20A GACAAGTTTGTACAAAAAAGCAGGCTTAGAAAACCTGTACT TCCAGGGTACGCCCGCGGCAGAAAGTCTG 3-pETG20-TssM 26Ct insertion of tssM (aa 868-1107) into pETG20A CCACTTTGTACAAGAAAGCTGGGTTTATTCGAAAACCGTTT CCGGCAGAAC RSF-Js-Fwd insertion of StrepII-tagged tssJ into pRSF-Duet ATGGTACATATGATGGCGATTATCGCTGGTAAGGCTGGTGG AATTCCATATGGCGATTATCGCTGGTAAGGCTGGTTACGGTC RSF-Js-Rev insertion of StrepII-tagged tssJ into pRSF-Duet AATCCGCTCGAGTCATTTTTCGAACTGCGGGTGGCTCCATTT ATCCTTCACCGGTAACAGGGTCAGGGTG RSF-flL-Fwd insertion of FLAG-tagged tssL into pRSF-Duet GCAGTTCGAAAAATGACTCGAGAAGGAGATATACCATGGA TTATAAAGATGACGATGACAAGAATAAACCTGTTATCTCCCGGGC RSF-flL-Rev insertion of FLAG-tagged tssL into pRSF-Duet ATGTATATCTCCTTTTATCCCTGCCCGGTAAGCCGTGCCACC TGGTCTG RSF-hM-Fwd insertion of 6×His-tagged tssM into pRSF-Duet GGATAAAAGGAGATATACATATGCATCACCATCATCACCACC ATCACAATAAACTGGCCTGTCTGTCTGGTC RSF-hM-Rev insertion of 6×His-tagged tssM into pRSF-Duet GCGGTTTCTTTACCAGACTCGAGTCAGTCAGTCTCCTCCACG GTATCC For site-directed mutagenesis e A-TssJ C1S Cys1-to-Ser substitution in tssJ TTATCAGGATCCGGTCTGACGCAAAGAGTGGCAGACGGTACGGTATCTGC B-TssJ C1S Cys1-to-Ser substitution in tssJ GTCAGACCGGATCCTGATAACGACAGGGAAAACAACGCAATAAT A-TssM-C727S Cys727-to-Ser substitution in tssM GAATACGCTGGCGGTTCAGGGATCCACTGGCCAGCCCCGGGAAG B-TssM-C727S Cys727-to-Ser substitution in tssM CTTCCCGGGGCTGGCCAGTGGATCCCTGAACCGCCAGCGTATTC A-TssM-T972C Thr972-to-Cys substitution in tssM ATAGCGGATGTGGCGTTCACCTGTGGTAACGCGGGGCTGCATTTTG B-TssM-T972C Thr972-to-Cys substitution in tssM CAAAATGCAGCCCCGCGTTACCACAGGTGAACGCCACATCCGCTAT A-TssM-V989C Val989-to-Cys substitution in tssM CGCCCGGGAACTGCTGCCGGTTGTATGCAGACGACGCTGATAAC B-TssM-V989C Val989-to-Cys substitution in tssM GTTATCAGCGTCGTCTGCATACAACCGGCAGCAGTTCCCGGGCG A-TssM-N1005C Asn1005-to-Cys substitution in tssM GATAATCAGAAACTGATTTATGTTTGTCAGATGCCGGTATGGAAGCG B-TssM-N1005C Asn1005-to-Cys substitution in tssM CGCTTCCATACCGGCATCTGACAAACATAAATCAGTTTCTGATTATC A-TssM-T1019C Thr1019-to-Cys substitution in tssM GATTTACCTGGCCGGCTGATTGTGAAGCACCTGGCGCCAGTTTAAG B-TssM-T1019C Thr1019-to-Cys substitution in tssM CTTAAACTGGCGCCAGGTGCTTCACAATCAGCCGGCCAGGTAAATC A-TssM-T1035C Thr1035-to-Cys substitution in tssM GGGTAAGCACTCAGGCCGTGTCCCGTCAGTATGCAGACCTGCCGGG B-TssM-T1035C Thr1035-to-Cys substitution in tssM CCCGGCAGGTCTGCATACTGACGGGACACGGCCTGAGTGCTTTACCC A-TssM-V1062C Val1062-to-Cys substitution in tssM GACGGAAAGCCGCACCGGTGTTTGCCAGTGGCTGGAGCCTGAG B-TssM-V1062C Val1062-to-Cys substitution in tssM CTCAGGCTCCAGCCACTGGCAAACACCGGTGCGGCTTTCCGTC A-TssM-G1075C Gly1075-to-Cys substitution in tssM CTGAGCTGGCAGGCGCAGGACTGTCGTATGCTGAATTACACACTGC B-TssM-G1075C Gly1075-to-Cys substitution in tssM GCAGTGTGTAATTCAGCATACGACAGTCCTGCGCCTGCCAGCTCAG A-TssM-V1092C Val1092-to-Cys substitution, tssM GGGGAAGGGCCGCTTTGTTTGCTGAAACTCCGCAATTTTG B-TssM-V1092C Val1092-to-Cys substitution in tssM CAAAATTGCGGAGTTTCAGCAAACAAAGCGGCCCTTCCCC A-TssM-S1109C Ser1109-to-Cys substitution in tssM GAAACGGTTTTCGAACTCTGCGGCACGTCAGCGTTTAC B-TssM-S1109C Ser1009-to-Cys substitution in tssM GTAAACGCTGACGTGCCGCAGAGTTCGAAAACCGTTTC a Sequences corresponding to the downstream and upstream regions of the gene to be deleted underlined. b Sequence annealing on the target plasmid underlined. c Restriction sites in Bold. d FLAG