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and bound proteins were collected and analyzed by SDS- PAGE and Coomassie blue staining. The different proteins are indicated on the right. Molecular weight markers (in kDa) are indicated on the left ,
Relative fluorescence levels (in arbitrary units) of E. coli cells carrying the indicated pUA66-promoter derivatives (GFP under the control of the indicated promoter) in absence (-, white bars) or presence (+, blue bars) or PorX. Is represented the mean of fluorescence levels obtained from three independent experiments (each measured in triplicate) The production of VSV-G-tagged PorX is shown on bottom (immunodetection with anti-VSV-G monoclonal antibody ,