HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Book sections

Chapter 23 Defining assembly pathways by fluorescence microscopy

Abstract : Bacterial secretion systems are amongst the largest protein complexes in prokaryotes, and display remarkably complex architectures. Their assembly often follows clearly defined pathways. Deciphering these pathways not only reveals how bacteria accomplish to build these large functional complexes, but can provide crucial information on the interactions and subcomplexes within secretion systems, their distribution within the bacterium, and even functional insights. The emergence of fluorescent proteins has provided a new powerful tool for biological imaging, and the use of fluorescently labeled components presents an interesting method to accurately define the biogenesis of macromolecular complexes. Here, we describe the use of this method to decipher the assembly pathway of bacterial secretion systems.
Complete list of metadata

Cited literature [27 references]  Display  Hide  Download

Contributor : Eric Cascales Connect in order to contact the contributor
Submitted on : Friday, April 27, 2018 - 6:15:53 PM
Last modification on : Wednesday, November 3, 2021 - 6:46:24 AM
Long-term archiving on: : Tuesday, September 25, 2018 - 9:03:37 AM


Files produced by the author(s)


  • HAL Id : hal-01780738, version 1



Abdelrahim Zoued, Andreas Diepold. Chapter 23 Defining assembly pathways by fluorescence microscopy. Methods Mol Biol , 2017. ⟨hal-01780738⟩



Record views


Files downloads