Serum microRNAs are altered in various stages of chronic kidney disease: a preliminary study

Benjamin Brigant (1) , Valérie Metzinger-Le Meuth (2, 1) , Ziad Massy (3, 4) , Nathalie Mckay (5) , Sophie Liabeuf (6) , Marion Pelletier (7) , Marion Sallée (5) , Eléonore M'Baya-Moutoula (1) , Pascale Paul (5) , Tilman B Drueke (3) , Stéphane Burtey (5) , Laurent Metzinger (1)

1 MP3CV - Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517
2 UFR SMBH - UFR Santé, Médecine et Biologie Humaine
3 CESP - Centre de recherche en épidémiologie et santé des populations
4 Hôpital Ambroise Paré [AP-HP]
5 VRCM - Vascular research center of Marseille
6 Centre de Recherche Clinique
7 Université Bordeaux Segalen - Bordeaux 2

Background: MicroRNAs (miRNAs) are innovative and informative blood-based biomarkers involved in numerous pathophysiological processes. In this study and based on our previous experimental data, we investigated miR-126, miR-143, miR-145, miR-155 and miR-223 as potential circulating biomarkers for the diagnosis and prognosis of patients with chronic kidney disease (CKD). The primary objective of this study was to assess the levels of miRNA expression at various stages of CKD. Methods: RNA was extracted from serum, and RT-qPCR was performed for the five miRNAs and cel-miR-39 (internal control). Results: Serum levels of miR-143, -145 and -223 were elevated in patients with CKD compared with healthy controls. They were further increased in chronic haemodialysis patients, but were below control levels in renal transplant recipients. In contrast, circulating levels of miR-126 and miR-155 levels, which were also elevated in CKD patients, were lower in the haemodialysis group and even lower in the transplant group. Four of the five miRNA species were correlated with estimated glomerular filtration rate, and three were correlated with circulating uraemic toxins. Conclusions: This exploratory study suggests that specific miRNAs could be biomarkers for complications of CKD, justifying further studies to link changes of miRNA levels with outcomes in CKD patients.

Mots clés: CKD; haemodialysis; microRNAs; renal transplantation; serum biomarkers

Domaines: Urologie et Néphrologie

Benjamin Brigant, Valérie Metzinger-Le Meuth, Ziad Massy, Nathalie Mckay, Sophie Liabeuf, et al.. Serum microRNAs are altered in various stages of chronic kidney disease: a preliminary study. Clinical Kidney Journal, 2017, 10 (1), pp.30-37. ⟨10.1093/ckj/sfw060⟩. ⟨hal-01790133⟩