Journal articles
Scrutiny of Mycobacterium tuberculosis 19 kDa antigen proteoforms provides new insights in the lipoglycoprotein biogenesis paradigm
Abstract : Post-translational modifications (PTMs) are essential processes conditioning the biophysical properties and biological activities of the vast majority of mature proteins. However, occurrence of several distinct PTMs on a same protein dramatically increases its molecular diversity. The comprehensive understanding of the functionalities resulting from any particular PTM association requires a highly challenging full structural description of the PTM combinations. Here, we report the in-depth exploration of the natural structural diversity of the M. tuberculosis (Mtb) virulence associated 19 kDa lipoglycoprotein antigen (LpqH) using intact protein high-resolution mass spectrometry (HR-MS) coupled to liquid chromatography. Combined top-down and bottom-up HR-MS analyses of the purified Mtb LpqH protein allow, for the first time, to uncover a complex repertoire of about 130 molecular species resulting from the intrinsically heterogeneous combination of lipidation and glycosylation together with some truncations. Direct view on the co-occurring PTMs stoichiometry reveals the presence of functionally distinct LpqH lipidation states and indicates that glycosylation is independent from lipidation. This work allowed the identification of a novel unsuspected phosphorylated form of the unprocessed preprolipoglycoprotein totally absent from the current lipoglycoprotein biogenesis pathway and providing new insights into the biogenesis and functional determinants of the mycobacterial lipoglycoprotein interacting with the host immune PRRs.
Cited literature [53 references]
https://hal-amu.archives-ouvertes.fr/hal-01791689
Contributor : Stephane Canaan Connect in order to contact the contributor
Submitted on : Monday, May 14, 2018 - 5:54:09 PM
Last modification on : Wednesday, November 3, 2021 - 9:57:26 AM
Long-term archiving on: : Monday, September 24, 2018 - 5:31:41 PM
Contributor : Stephane Canaan Connect in order to contact the contributor
Submitted on : Monday, May 14, 2018 - 5:54:09 PM
Last modification on : Wednesday, November 3, 2021 - 9:57:26 AM
Long-term archiving on: : Monday, September 24, 2018 - 5:31:41 PM
File
Parra-SciRep-2017.pdf
Publisher files allowed on an open archive