GH10 xylanase D from Penicillium funiculosum: biochemical studies and xylooligosaccharide production

Mickael Lafond (1) , Alexandra Tauzin (2) , Véronique Desseaux (3) , Estelle Bonnin (4) , El-Hassan Ajandouz (1) , Thierry Giardina (1)

1 ISM2 - Institut des Sciences Moléculaires de Marseille
2 TWB - Toulouse White Biotechnology
3 PNP - Physiologie neurovégétative - PNV
4 BIA - Unité de recherche sur les Biopolymères, Interactions Assemblages

Background: The filamentous fungus Penicillium funiculosum produces a range of glycoside hydrolases (GH). The XynD gene, encoding the sole P. funiculosum GH10 xylanase described so far, was cloned into the pPICZaA vector and expressed in methylotrophe yeast Pichia pastoris, in order to compare the results obtained with the P. funiculosum GH11 xylanases data. Results: High level expression of recombinant XynD was obtained with a secretion of around 60 mg.L-1. The protein was purified to homogeneity using one purification step. The apparent size on SDS-PAGE was around 64 kDa and was 46 kDa by mass spectrometry thus higher than the expected molecular mass of 41 kDa. The recombinant protein was N-and O-glycosylated, as demonstrated using glycoprotein staining and deglycosylation reactions, which explained the discrepancy in molecular mass. Enzyme-catalysed hydrolysis of low viscosity arabinoxylan (LVAX) was maximal at pH 5.0 with Km (app) and k cat /Km (app) of 3.7 ± 0.2 (mg.mL-1) and 132 (s-1 mg-1 .mL), respectively. The activity of XynD was optimal at 80°C and the recombinant enzyme has shown an interesting high thermal stability at 70°C for at least 180 min without loss of activity. The enzyme had an endo-mode of action on xylan forming mainly xylobiose and short-chain xylooligosaccharides (XOS). The initial rate data from the hydrolysis of short XOS indicated that the catalytic efficiency increased slightly with increasing their chain length with a small difference of the XynD catalytic efficiency against the different XOS. Conclusion: Because of its attractive properties XynD might be considered for biotechnological applications. Moreover, XOS hydrolysis suggested that XynD possess four catalytic subsites with a high energy of interaction with the substrate and a fifth subsite with a small energy of interaction, according to the GH10 xylanase literature data.

Mots clés: xylanase GH10; Penicillium funiculosum; xylooligosaccharides

Domaines: Biochimie, Biologie Moléculaire

Mickael Lafond, Alexandra Tauzin, Véronique Desseaux, Estelle Bonnin, El-Hassan Ajandouz, et al.. GH10 xylanase D from Penicillium funiculosum: biochemical studies and xylooligosaccharide production. Microbial Cell Factories, 2011. ⟨hal-01793303⟩