Skip to Main content Skip to Navigation
Journal articles

Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species.

Abstract : In order to evaluate the suitability of 16S rRNA nucleotide sequence similarity for the classification of new Mycobacterium isolates at the species level, we systematically studied the pairwise identity values of this gene for 131 Mycobacterium species with standing in nomenclature. Only one of the studied species, M. poriferae (0.76%), strictly respected the 95% and 98.65% threshold values currently recommended to determine the affiliation of bacterial isolates to an existing or new genus or species, respectively. All other species exhibited at least an identity value \textgreater98.65% and/or \textless95% with another Mycobacterium species. Therefore, we suggest that interpretation of interspecies 16S rRNA identity values should be made cautiously when classifying a new mycobacterial isolate at the species level.
Complete list of metadatas

Cited literature [21 references]  Display  Hide  Download

https://hal-amu.archives-ouvertes.fr/hal-01795912
Contributor : Administrateur Hal Amu <>
Submitted on : Friday, June 1, 2018 - 2:59:01 PM
Last modification on : Tuesday, April 16, 2019 - 4:53:49 PM
Long-term archiving on: : Sunday, September 2, 2018 - 1:50:39 PM

File

Careful use.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Mamadou Beye, Nidal Fahsi, Didier Raoult, Pierre-Edouard Fournier. Careful use of 16S rRNA gene sequence similarity values for the identification of Mycobacterium species.. New Microbes and New Infections, Wiley Online Library 2018, 22, pp.24-29. ⟨10.1016/j.nmni.2017.12.009⟩. ⟨hal-01795912⟩

Share

Metrics

Record views

181

Files downloads

352