Localization of the Gene Encoding Peptidylglycine α-Amidating Monooxygenase (PAM) to Human Chromosome 5q14-5q21

L'Houcine Ouafik (1) , Marie Geneviève Mattei (1) , Pierre Giraud (1) , Charles Oliver (1) , Betty Eipper (2) , Richard Mains (2)

1 INSERM U 297 - Laboratoire de Neuroendocrinologie Expérimentale
2 JHU - Johns Hopkins University

Peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is a multifunctional protein containing two enzymes that act sequentially to catalyze the alpha-amidation of neuroendocrine peptides. Southern blot analysis of human placental DNA demonstrated that PAM is encoded by a single gene. The chromosomal localization of the PAM gene was established using in situ hybridization. A 2.2-kb human PAM cDNA hybridized to human metaphase chromosomes revealed a significant clustering of silver grains over chromosome 5 bands q14-q21. The gene encoding another enzyme important in the post-translational processing of neuroendocrine precursors, prohormone convertase 1 (PC1), is localized in the same region (5q15-q21).

Domaines: Cancer

L'Houcine Ouafik, Marie Geneviève Mattei, Pierre Giraud, Charles Oliver, Betty Eipper, et al.. Localization of the Gene Encoding Peptidylglycine α-Amidating Monooxygenase (PAM) to Human Chromosome 5q14-5q21. Genomics, 1993, 18 (2), pp.319 - 321. ⟨10.1006/geno.1993.1471⟩. ⟨hal-01807998⟩